To determine if this were the case on our task, we used an independent face–place localizer (see the subsection “Face–Place Localizer” in the “Methods” section) to identify participant-specific face- and place-selective fROIs (Fig. 4a). Neither fROI showed significant encoding of the ...
ATPase assays were performed at 30 °C in the Activity Buffer containing ClpA6 variants (0.1 μM), ClpP14 (0.3 μM), plus or minus ClpS (0.1 μM when present). The hydrolysis of 5 mM ATP (Sigma-Aldrich) was measured using an NADH-coupled assay (56) with an ATP-regeneration system...
We used BLOSUM Clustered Scoring Matrix to obtain extended 7-mers when counting the occurrence of a specific 7-mer seed to maximize the flexibility of a potential functional protein site (i.e., allowing for D/E at the same position; STAR Methods) (Henikoff and Henikoff, 1992). GQFDDAD ...
we determined the surface expression of the CLRs MMR (Macrophage Mannose Receptor, CD206)28and DC-SIGN29(Dendritic-Cell Specific Intercellular adhesion molecule-3-Grabbing Non-integrin, CD
These lipids must then be transported across the chloroplast outer envelope membrane to the inner envelope membrane, where they are further metabolized into chloroplast-specific lipids, such as monogalactosyldiacylglycerol (11), for eventual transport to the thylakoids. Alternatively, chloroplasts can ...