However, more important than quantity is the discriminatory power of the markers that are used. Here we review how improved combinations of commonly used markers during multicolor flow cytometry, followed by in‐depth functional assays, have been used to identify novel human B cell populations and ...
1). We determined cellular gates based on empiric distributions of blood cells under perturbation conditions and defined parameter sets for all observed cell populations (Fig. 1b and Extended Data Fig. 2). The perturbation conditions represented discrete classes of exposure likely to contribute to ...
Expression deconvolution: a reinterpretation of DNA microarray data reveals dynamic changes in cell populations. Proc. Natl. Acad. Sci. USA 100, 10370–10375 (2003). Article CAS Google Scholar Zhong, Y. & Liu, Z. Gene expression deconvolution in linear space. Nat. Methods 9, 8–9 (2012)...
Isotypes of CD45 have been used extensively as markers of memory and naive populations of T cells in peripheral blood. In this study, T cells were isolated... S Wilamasundera,DRK And,BM Chain - 《European Journal of Immunology》 被引量: 34发表: 1998年 CD57+ T lymphocytes are derived ...
(RNA-seq) was conducted on sorted populations. The mRNA content of each sorted subset group was assessed for shared and unique transcripts defining MTC from naïve T cells. Relative to their naïve counterparts, TCMand TEMcells exhibited 805 and 1,486 differentially expressed genes (DEG), ...
Although Langerhans cells are probably related to the Ia-like antigen positive dermal cells only a few of the abundant latter population were HTA-1+. In the remaining 2 cases, larger populations of OKT8+ (suppressor/cytotoxic) cells were seen and could be heralding a particularly benign course...
Pathogen-sensing machinery expression in human DC populations in the human myeloid scRNA atlas.AVisualization of PhenoGraph clusters on an MNP-VERSE UMAP plot following the coordinates presented by Mulder et al., 2021. Briefly, MNP-VERSE was downloaded fromhttps://github.com/gustaveroussy/FG-Lab,...
Simultaneous selection of extreme populations : Bayes-p and minimax rules Let i denote a population characterized by an unknown parameter i, i=1,,k ( 2). Populations associated with [1] = min i and [k] = max i are called the lowe... N Misra,ID Dhariyal - 《Communications in Statist...
moDCs are the most controversial of the three populations. However, given their use in many human-based cancer immunotherapies2 they cannot be excluded from discussion. They are routinely generated in vitro following culture of monocytes from both human and mouse with granulocyte-macrophage colony ...
than 6 million distinct 25mer oligonucleotide probes were used for the profiling analysis of whole RNA prepared from blood samples of 10 RRMS patients at 3 different time points (before as well as ~24 h and 3 months after the start of fingolimod therapy) for each of the 5 cell populations...