Cyanophycin is a natural biopolymer produced by a wide range of bacteria, consisting of a chain of poly-l-Asp residues with l-Arg residues attached to the β-carboxylate sidechains by isopeptide bonds. Cyanophycin is synthesized from ATP, aspartic acid and arginine by a homooligomeric enzyme ...
For the first time, correlations between THz spectral peaks and the molecular structures of amino acids are revealed and a classification of amino acids based on both molecular structures and THz spectra was established. These correlations can help identify amino acids, trace some functional groups,...
the first and rate-limiting enzyme of the MEP-pathway18. DXPS catalyzes the ThDP-dependent decarboxylation of pyruvate and subsequent carboligation withd-glyceraldehyde-3-phosphate (D-GAP), the second substrate, following a preferred-order, random-sequential reaction mechanism (Fig....
被引量: 20发表: 2009年 Chiral and water-soluble poly(2-oxazoline)s We describe the synthesis and characterization of the first water-soluble and chiral poly(2,4-disubstituted-2-oxazoline)s. While poly(2,4-dimethyl-2-oxazol... Robert,Luxenhofer,Stephan,... - 《Journal of Polymer Science ...
For the first time, correlations between THz spectral peaks and the molecular structures of amino acids are revealed and a classification of amino acids based on both molecular structures and THz spectra was established. These correlations can help identify amino acids, trace some functional groups,...
Previous studies have indicated that the second Tudor domain is capable of binding methylated resides on histone tails [16], while no such function has been ascribed to the first Tudor domain. In this work, we report the crystal structures of the two N-terminal Tudor domains of PHF20. We ...
MCLs are known to function in different pathways of central carbon metabolism in Bacteria and Haloarchaea. The very first MCLs were described in Methylobacterium extorquens AM1 (formerly Pseudomonas sp. AM1) [12, 16] and Aminobacter aminovorans (formerly Pseudomonas sp. MA) [17]. In ...
Since the first crystal structure of aspartate aminotransferase (AAT; Ref. 9), three-dimensional structures of PLP-dependent enzymes have been determined at an explosive speed in the last decade, and over 200 crystal structures have been deposited in the Protein Data Bank to date. The structures...
However, we found that the ligase active site lysine (K568) stays adenylated (LIG1-AMP) while engaging with a A:C mismatch, which refers to the first ligation step. Furthermore, we showed mutagenic and defective nick sealing of 3′-G:T and 3′-A:C mismatches, respectively. Finally, ...
The world is currently undergoing a pandemic caused by an H1N1 influenza A virus, the so-called 'swine flu'. The H5N1 ('bird flu') influenza A viruses, now circulating in Asia, Africa and Europe, are extremely virulent in humans, although they have not s