The tools of viral structural biology • Electron microscopy • X-ray crystallography • Cryo-electron microscopy (cryoEM) & cryo-electron tomography • Nuclear magnetic resonance spectroscopy (NMR) 展示了几种常见病毒的模拟结构彩图,如Polioviru...
The Structure & Complexity of Virus Genomesmore varied than:结构&;病毒genomesmore比变化的复杂性of,结构,帮助,the,Col,The,than,virus 文档格式: .ppt 文档大小: 662.0K 文档页数: 36页 顶/踩数: 0/0 收藏人数: 0 评论次数: 0 文档热度:
Virus Structure Tutorial STEM IntroductiontoVirusStructure Tutorial JonathanKing,PeterWeigele,GregPintilie,DavidGossard(MIT)v.November,2008 VirusStructure ••Size –17nm–3000nmdiameter Basicshape ––Rod-like“Spherical”• ProtectiveShell-Capsid –Madeofmanyidenticalproteinsubunits–Symmetricallyorganized...
Moloney murine leukemia virus (MoMLV)Reverse transcriptionPolypurine tract (PPT)This review compares the well-studied RNase H activities of human immunodeficiency virus, type 1 (HIV-1) and Moloney murine leukemia virus (MoMLV) reverse transcriptases. The RNase H domains of HIV-1 and MoMLV are ...
It is 1016 kDa as the trimer based on the calculated molec- ular weight and has a theoretical isoelectric point of 4.76. For the site-specific cleavage of the PSD, we selected human rhinovirus 3C (HRV 3C) protease, which recognizes a unique amino acid sequence with high specific- ity....
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tuberculosis and a tobacco etch virus (TEV) protease site to replace the thrombin site coding sequence (named pET-15b-Tev). This culture was grown at 37°C, and then the cells were harvested by centrifugation and the resulting cell pellet was resuspended in buffer A [50 mM Tris-HCl (pH ...
A specific terminal structure of preintegrative DNA is required for transposition of retroviruses and LTR-retrotransposons. We have used an anchored PCR technique to map the 3'ends of DNA intermediates synthesized inside yeast Ty1 and Ty3 retrotransposon virus-like particles. We find that, unlike ...
The present invention improves in vitro virus synthesis efficiency and stability of mRNA derived from screened cDNA in a cDNA display method to improve the efficiency and reliability of the production of a peptide by a molecular evolutionary engineering technique. Provided is a ligand which comprises ...
The aim of this study was to investigate the genetic diversity in the stem-loop (SL) IV sub-domain of the human rhinovirus (HRV) internal ribosomal entry site (IRES), which plays key roles in the initiation of viral translation by host protein interaction. Methods The primary SL-IV sequenc...