A mechanism of the phosphorolysis of tRNA by this enzyme is proposed. The model suggests that the enzyme, once it attacks a molecule of tRNA degrades it completely before leaving it. This mechanism has been extended to other polynucleotides. The extent of phosphorolysis of tRNA changes ...
The three dimensional structure of the tRNA molecule is not a cloverleaf, the molecule instead folds back on itself, forming two segments of double helix and most of the upaired bases in the T and D loops form base pairs with each other (FIGURE 46). One of the two segments of double h...
Here we report the 3.2-A-resolution structure of the tRNA-like domain of tmRNA (tmRNA(Delta)) in complex with small protein B (SmpB), a protein essential for biological functions of tmRNA. We find that the flexible RNA molecule adopts an open L-shaped conformation and SmpB binds to its ...
The molecule associated with the N-terminal lobe was convincingly modeled as coenzyme A (CoA) based on: (i) the density of the terminal ppAp moiety in the Tpt1 active site and the anomalous difference peaks overlying the phosphorus atoms of the terminal pAp moiety (Fig.2c), (ii) the ele...
跟DNA比起来,RNA分子是比较短的片段,一般从几倍到上千个核苷酸长。它是单链结构,骨架中的五碳糖是核糖,碱基则是GACU,U替代了DNA中的T。RNA的种类有三种,mRNA(messenger RNA)存在于细胞核和细胞质中,tRNA(transfer RNA)和rRNA(ribosomal RNA)存在于细胞质中。RNA可以通过核孔,DNA则不行。练习题链接...
The process of RNA editing can occur in various types of RNA molecules, including pre-mRNA, mature mRNA, miRNA, lncRNA, tRNA, and even viral RNA [11,12,13]. An important impact of RNA editing is its ability to challenge the central dogma of biology. When RNA editing takes place in th...
When two or more dyes are present on a nucleic acid molecule, it may be possible to determine the distance between them by energy transfer measurements ( Stryer and Haugland , 1967). If the dyes are attached at known points in the primary structure of the nucleic acid, the resulting ...
This chapter describes the procedures involved in the determination of a crystal structure of a tRNA by crystallographic method. Determination of the crystal structure of a transfer RNA involves four principal steps: (a) crystallization of the molecule, (b) collection of X-ray diffraction data from...
Gallie DR, Feder JN, Schimke RT, Walbot V: Functional analysis of the tobacco mosaic virus tRNA-like structure in cytoplasmic gene regulation. Nucl Acids Res 19: 5031–5036 (1991). PubMedCASGoogle Scholar Garcia A, Cuin JV, Pleij CWA: Differential response to frame shift signals in eukary...
The crystallographic structure of the synthetic oligoribonucleotide, U(UA) 6A, has been solved at 2.25 resolution. The crystallographic refinement permitted the identification of 91 solvent molecules, with a final agreement factor of 13%. The molecule is a dimer of 14 base-pairs and shows the typ...