These differences in the intercistronic regions may reflect functional relationships between the products of adjacent genes in the operon.ChristiePlattCHRISTIEG, . E., and T. PLATT.1980. Gene structure in the tryptophan operon of Escherichia coli: nucleotide sequence of trpC and the flanking inter...
The solution structure of a B-DNA undecamer comprising a portion of the specific target site for the cAMP receptor protein in the gal operon. Refinement on... A restrained least squares refinement of the solution structure of the double-stranded DNA undecamer 5'd(AAGTGT-GACAT).5'd(ATGT...
The Myoviridae cyanophage A-1(L) specifically infects the model cyanobacteria Anabaena sp. PCC 7120. Following our recent report on the capsid structure of A-1(L), here we present the high-resolution cryo-EM structure of its intact tail machine including
Figure 1. Organisation of nonribosomal peptide synthetases, sequence characteristics of PCPs and modification of PCP. (a) Top: the tyc operon from B. brevis is shown as an example of an NRPS gene. The operon contains three genes, tycA, tycB and tycC; red bars represent the adenylation (A...
Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination Transcription termination at the attenuators of the trp operons of Escherichia coli and Salmonella typhimurium was studied in vitro using DNA ...
The B. subtilis tryptophan RNA-binding attenuation protein (TRAP; [9]) controls transcription of the trp operon in response to changes in the intracellular levels of tryptophan. Like UMP-bound PyrR, tryptophan-bound TRAP induces formation of an mRNA stem-loop terminator in the control region of...
despite sharing a β-helix fold, the overall structure of the C-terminal catalytic domain of TSP3 is quite different when compared to TSP1. The TSP3 structure suggests that the glycosidase active site resides in a cleft at the interface between two adjacent subunits where three acidic residues...
Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often plasmid DNAs, from a donor cell to a recipient cell1. It is the primary means by which antibiotic resistance genes spread among bacterial populations2,3. In Gram-n
A kinetic analysis of the TRAP-RNA interaction of wild-type and mutant proteins with different RNAs would provide valuable insight into the regulation of thetrp operon. In vitro selection (SELEX) studies to characterize the role of Arg-58 in determining specificity in the TRAP-RNA interaction ...
The Trp zipper between Trp-267 and Trp-274 of the β-ribbon are show as ball-and-stick representation. The K-domain of C. freundii has the same structure as the DhaK subunit of E. coli kinase (33). It consists of two α/β-folds, each containing a six-stranded mixed β-sheet ...