There is increasing interest in the roles of covalently modified nucleotides in RNA. There has been, however, an inability to account for modifications in secondary structure prediction because of a lack of software and thermodynamic parameters. We repor
Proper subcellular localization is crucial for the functioning of biomacromolecules, including proteins and RNAs. Nuclear transport is a fundamental cellular process that regulates the localization of many macromolecules within the nuclear or cytoplasmic compartments. In humans, approximately 60 proteins are i...
It is pointed out that the structure preference of codons is related to the distribution of mRNA stem/doi:10.1002/bip.20094Liaofu LuoDepartment of PhysicsMengwen JiaDepartment of PhysicsXiaoqin LiDepartment of PhysicsBiopolymersLF Luo,MW Jia,and XQ Li.Protein Structure Preference,tRNA Copy Number ...
including pre-mRNA, mature mRNA, miRNA, lncRNA, tRNA, and even viral RNA [11,12,13]. An important impact of RNA editing is its ability to challenge the central dogma of biology. When RNA editing takes place in the coding sequence (CDS) region of an mRNA, where an I is recognized as...
(rRNA) scaffold that sustains the ribosome24. Importantly, the structure of a messenger RNA (mRNA) defines its lifecycle25, recruitment of ribosomes and response against environmental changes25. There are several cases of nucleotide chains of non-coding RNAs acting as scaffolds for protein complexes...
Compare the tRNA and mRNA in terms of their functions and how they are synthesized within the cell. Give three separate analogies for the following: DNA replication, RNA transcription, and protein synthesis. How is the structure of DNA different from the structure of RNA? Draw the specific str...
The GTP-bound form of the trimeric eukaryotic translation initiation factor 2 (eIF2) transfers aminoacylated initiator methionyl tRNA onto the 40S ribosome. We have solved with solution NMR the structure of the α subunit of human eIF2 (heIF2α). The protein consists of two domains that are...
of the most eminent examples is the class of microRNAs [1,2], an abundant class of small functional RNAs that regulate gene expression by binding to a target in the mRNA. Other examples include snoRNAs, which modify ribosomal RNA [3],signal recognition particleRNAs [4],cis-acting regulatory...
The location of the C-terminal domain (CTD) clarifies its anti-association activity, whereas the orientation of the mito-specific CTE provides a mechanistic explanation for its role in destabilizing initiator tRNA in the absence of mRNA. Furthermore, our structure hints at a possible role of ...
these advances allow the prediction of RNA secondary structures for sequences with m6A. We demonstrate, for calculations with human mRNA sequences known to contain m6A, that N6-methylation alters the folding landscape so that m6A is less likely to be buried in a helix, i.e., stacked between...