Crystal structure of isoamyl acetate‐hydrolyzing esterase from Saccharomyces cerevisiae reveals a novel active site architecture and the basis of substrate specificity. Proteins: Struct, Funct, Bioinf. 2011;79(2):662-8.Ma, J.; Lu, Q.; Yuan, Y.; Ge, H.; Li, K.; Zhao, W.; Gao, Y....
6.Synthesis of Octahedral Molecular Sieve and Selective Oxidation of Toluene to Benzaldehyde;八面体分子筛(OMS-2)的合成及选择性氧化甲苯制苯甲醛研究 7.Preparation of OMS-2 Based Solid Acid under Microwave Irradiation and Its Application in Synthesis of Isoamyl Acetate微波固相法制备OMS-2基固体酸及其催...
After centrifugation, the supernatant was gently extracted twice with 24:1 chloroform:isoamyl alcohol. The upper phase was transferred to a new tube and 1/10th volume of 3 M sodium acetate was added, the solution gently mixed and DNA precipitated with isopropanol. The DNA precipitate was ...
Telomerase is a ribonucleoprotein (RNP) enzyme that extends telomeric repeats at eukaryotic chromosome ends to counterbalance telomere loss caused by incomplete genome replication. Human telomerase is comprised of two distinct functional lobes tethered b
(Sigma-Aldrich, United States), 100 mM TrisHCl (pH 7.4), 20 mM EDTA (pH 8), 800 mM ammonium acetate] with 1% SDS. The mixture was incubated at 65 °C for 2 h and placed on ice for 30 min. The DNA was then purified with phenol–chloroform-isoamyl alcohol (25:24:1), followed...
(2) The resulting supernatant was centrifuged, and then an equal volume of phenol (pH 8.0): chloroform: isoamyl alcohol (25:24:1) was added. The mixture was inverted and centrifuged at 12,000 rpm for 10 min. (3) An equal volume of chloroform: isoamyl alcohol (24:1) was added to ...
than on the nucleolus itself. In the present study, we identified a lncRNA (transcript ID: ENST00000564237.1, gene name: RP11-196G18.22) that directly controls the structural organization and major functions of the nucleolus via a key nucleolar protein NPM1 (also named B23 or nucleophosmin)...
DNA fragments were purified by extraction with phenol/chloroform/isoamyl alcohol (25:24:1, pH 7.9, Nacalai Tesque) and MaXtract High Density (QIAGEN). DNA fragments were precipitated with 1/1250 volume of GlycoBlue (Ambion), 1/10 volume of 3 M sodium acetate and the same volume of ...
The plasmid was recovered from yeast after breaking cells by vortexing in the presence of glass beads in TNSTE-PCI (1% Triton X-100, 0.5% SDS, 50 mM NaCl, 5 mM Tris pH 8.0, 0.5 mM EDTA, 50% phenol:- chloroform:isoamyl alcohol 25:24:1), then ethanol precipitating the aqueous phase...
isoamyl alcohol (24:1, v/v) followed by centrifugation. This step was repeated twice to avoid contamination. The DNA in solution was subsequently precipitated with 3M sodium acetate, pH 5.2 (1/10 volume) and 2/3 volume isopropanol stored at room temperature. Then, the DNA pellet was washed...