The structure of the aerogels depends on the nature of the solvent used to perform the hypercritical solvent evacuation. Infrared spectroscopy shows that an esterification reaction occurs during the methanol supercritical drying. The samples prepared by CO 2 supercritical drying retain some isoamylacetate...
After centrifugation, the supernatant was gently extracted twice with 24:1 chloroform:isoamyl alcohol. The upper phase was transferred to a new tube and 1/10th volume of 3 M sodium acetate was added, the solution gently mixed and DNA precipitated with isopropanol. The DNA precipitate was ...
42,43,44], and some researchers found that the coral-macroalgal shift did not influence the diversity, richness or variability of fish gut bacteriomes following climate-driven regime shifts. Additionally, some results showed the connectivity of a shared microbiome between seagrass, fish and...
Aquaculture, defined as the rearing or cultivation of aquatic organisms using techniques designed to increase their production beyond the natural capacity of the environment [1], is one of the pillars of sustainable food supply and its production has increased notably over the years [2]. With a ...
with an equal volume of phenol:choloroform:isoamyl alcohol (25:24:1 v/v/v; pH 5.5) before nucleic acids were precipitated with 100% isopropanol and 3 M sodium acetate and washed with 70% ethanol. The RNA was loaded on an Illustra Autoseq G-50 Spin Column (GE Healthcare, Pittsbur...
DNA fragments were purified by extraction with phenol/chloroform/isoamyl alcohol (25:24:1, pH 7.9, Nacalai Tesque) and MaXtract High Density (QIAGEN). DNA fragments were precipitated with 1/1250 volume of GlycoBlue (Ambion), 1/10 volume of 3 M sodium acetate and the same volume of ...
Our Invention relates to thio-barbituric acid derivatives, having the structure: R Co-NH R' bO-k-X in which R and R' represent saturated or unsaturated alkyl or aryl groups, either the same or different, at least one of which contains more 10 than three carbon atoms, and X represents ...
aInfluence of glucose and oxygen on the production of ethyl acetate and isoamyl 葡萄糖和氧气的影响对乙酸乙酯的生产和异戊基 [translate] aWho say the ain't got no jokes? They think if its called "South China Sea" it belongs to the Chinese. 谁认为没得到笑话? 他们认为它的叫的“南中国海”...
0.1 M sodium phosphate buffer (pH 8.0) and 0.5 ml The two burrow types shown in Figure 1 are of phenol/chloroform/isoamyl alcohol (25:24:1; generalisations. In this study, U. deltaura burrows pH 4.0). Samples were lysed using 0.3 g each of had between two and three entrance or ...
Examples of esters include ethyl acetate, n-butyl acetate, isobutyl acetate, cyclohexyl acetate, amyl formate, isoamyl acetate, butyl propionate, isopropyl butyrate, ethyl butyrate, butyl butyrate, methyl lactate, ethyl lactate, alkyl alkyloxyacetate esters (for example, methyl alkyloxyacetate, ethyl ...