(Gibco), at 37 °C and 5% CO2. CEM cells at 3 × 106cells/ml after 10 passages were pelleted at 200 x g for 5 min at 20 °C and resuspended in PBS mixed with 10% glycerol. An aliquot of 3 µl cell suspension was applied to the glow-discharged holey carbon...
7A, B). The reactions catalyzed by these two enzymes are directly related to the biosynthesis of glycerol 1-phosphate or glycerol 3-phosphate, which are the divergent precursors for the biosynthesis of archaeal or bacterial lipids, respectively65,66,67. Therefore, these two enzymes still had ...
This grouping is based on structural characteristics, especially those related to the mechanism of enzymatic catalysis. Although, both act on similar substrates, the GH1 β-glucosidases use a Glu residue as the catalytic nucleophile, whereas the GH3 β-glucosidases use an Asp residue as the ...
The labeled DNA was purified by two rounds of ethanol precipitation. 1 μl labeled and purified DNA was mixed with different concentrations of Synechocystis cryptochrome DASH in 20 μl buffer (50 mM Tris/HCl, 50 mM NaCl, 5% glycerol, 0.1 mM EDTA, 1 mM DTT, and 0.05 mg/ml bovine serum...
,Gierl, A.摘要: The indole-3-glycerol phosphate lyase Igl is the structural gene of volatile indole biosynthesis in the tritrophic interaction in maize. The gene is activated on transcriptional level with the same kinetics and to the same level by the fatty acid-amino acid conjugates (FAC's...
The cells were harvested at 8000 rpm for 10 min, and the cell pellets were resuspended in lysis buffer (50 mM Tris pH 7.5, 10% glycerol, 300 mM KCl, 5 mM DTT, 1 mM EDTA, 1 mM PMSF and 5 μg/ml each of chymostatin, leupeptin, and pepstatin). The cell lysates were frozen at ...
Regardless of the differences observed in their expression levels (Additional file 4A), the mutant proteins were quite stable up to 4-6 weeks when stored at 4°C in the assay buffer containing 10% glycerol. Table 2 Significance of the putative residues of TaOMT2 involved in binding ans/or ...
Cambridge, UK. Soy lecithin, cholesterol, peptone, beef extract, agar and yeast powder were of biological reagent. N-octane, Tween 80, Trition X-100, methylene blue, acid orange II, ZnCl2, KCl, NaCl, MgSO4, NaOH, ethanol, glycerol, and hydrochloric acid were of analytical reagent grade. ...
[24]. Triplicate samples of three independent batches of dECM hydrogel were solubilized in 8 M urea and 20 mM HEPES containing Na3VO4 (100 mM), NaF (0.5 M), β-glycerol phosphate (1 M), and Na2H2P2O7 (0.25 M) at 80 μg of protein for 300 µL of lysis buffer. Samples ...
The crystal structure of the β′-2 form of a mixed chain triacylglycerol (TAG), 1,2-dipalmitoyl-3-myristoyl- sn -glycerol (PPM), was determined to a final reliability factor of 0.074. This work is the first to resolve the atomic-level structure of the β′ polymorph, which is of the...