PhaA (i.e., thiolase) is the first enzyme in the PHA biosynthetic pathway, which catalyze the condensation of two acetyl-CoA molecules to acetoacetyl-CoA. Our study elucidated the crystal structure of PhaA in Bacillus cereus ATCC 14579 (BcTHL) in its apo- and CoA-bound forms. BcTHL ...
Molecular structure of Acetyl-Coa - important biomolecule 3D rendering,站酷海洛,一站式正版视觉内容平台,站酷旗下品牌.授权内容包含正版商业图片、艺术插画、矢量、视频、音乐素材、字体等,已先后为阿里巴巴、京东、亚马逊、小米、联想、奥美、盛世长城、百度、360、
The NDP-forming acyl-CoA synthetases (ACDs) catalyze the conversion of various CoA thioesters to the corresponding acids, conserving their chemical energy in form of ATP. The ACDs are the major energy-conserving enzymes in sugar and peptide fermentation of hyperthermophilic archaea. They are consid...
The unstable linear chromosome of Streptomyces lividans was circularized by homologous recombination and its terminal inverted repeats deleted. Strains wit... JN Volff,P Viell,J Altenbuchner - 《Mol Gen Genet》 被引量: 94发表: 1997年 Cloning of a Gene Cluster Encoding Enzymes Responsible for the...
BRISSE 147 A survey of the saturated 7-1actones in a variety of molecules reveals a common conformation in which the C , ~ - C O - O - C v group is planar and the fl-C atom is at the flap of this envelope conformation. This usual arrangement is found for example in 19-acetyl...
In the eukaryotic unicellular organism Trichomonas vaginalis a key step of energy metabolism, the oxidative decarboxylation of pyruvate with the formation of acetyl-CoA, is catalyzed by the iron-sulfur protein pyruvate:ferredoxin oxidoreductase (PFO) and not by the almost-ubiquitous pyruvate dehydrogenase...
Acetyl-CoA carboxylase-α (ACC-α) is a key enzyme in the regulation of fatty acid synthesis and is subject to both acute control, via reversible phos- phorylation, and chronic control that results in the reg- ulation of synthesis of the enzyme. The gene for ACC- α is expressed ...
Here, we present the X-ray crystal structure of the human αTAT1/acetyl-CoA complex. Together with structure-based mutagenesis, enzymatic analysis, and functional studies in cells, we elucidate the catalytic mechanism and mode of tubulin-specific acetylation. We find that αTAT1 has an overall ...
Inhibition of acetyl-CoA carboxylases (ACCs), a crucial enzyme for fatty acid metabolism, has been shown to promote fatty acid oxidation and reduce body fat in animal models. Therefore, ACCs are attractive targets for structure-based inhibitor design, particularly the carboxyltransferase (CT) ...
The reactions were initiated by adding 100 μM acetyl-CoA. After incubation at 37 °C for 10-80 min, the reactions were stopped by adding SDS loading buffer and heating at 95 °C for 5 min. Proteins were then resolved by 6–15% SDS-PAGE, followed by a western blot with ...