The Sec61/SecY channel allows the translocation of many proteins across the eukaryotic endoplasmic reticulum membrane or the prokaryotic plasma membrane. In bacteria, most secretory proteins are transported post-translationally through the SecY channel by the SecA ATPase. How a polypeptide is moved thr...
Here, we report the crystal structures of active ligand-free MHETase and MHETase bound to a nonhydrolyzable MHET analog. MHETase, which is reminiscent of feruloyl esterases, possesses a classic α/β-hydrolase domain and a lid domain conferring substrate specificity. In the light of structure-...
RESULTS: The crystal structure of UDP-N-acetylenolpyruvylglucosamine reductase, MurB, has been solved at 3.0 A and compared with our previously reported structure of MurB complexed with its substrate enolpyruvyl-UDP-N- acetylglucosamine. Comparison of the liganded structure of MurB with this un...
Abstract—This critical review considers the fundamental concept, methods of obtaining, stability, and properties of a new class of materials—high-entropy alloys and compounds. An analysis of factors that determine the structure and stability of multicomponent disordered solid solutions has been carried...
The organic layer was deposited onto the p-Si substrate to obtain an Al/p-si/phosphazene (HCP-Co)/Al diode. The current (I)- voltage (V) characteristics of heterojunction diode was measured under dark and illuminated conditions (20–100 mW/cm2) at room temperature. The electrical ...
RT-QuIC assay for DLB samples were performed using the recombinant αSyn K23Q substrate purified using a two-step chromatography protocol described previously (PMID: 29422107). For testing MSA samples, wild-type αSyn recombinant substrate was purified using anion-exchange and size exclusion chromatogr...
A new class of phospholipases A2 with lysine in place of aspartate 49. Functional consequences for calcium and substrate binding J. Biol. Chem., 259 (1984), pp. 13839-13843 View PDFView articleView in ScopusGoogle Scholar [6] A.M. Soares, W.P. Sestito, S. Marcussi, R.G. Stabeli...
The major differences in electromechanical coupling reflect the functional distinction between channels and transporters—channels conduct a multitude of ions in a single open state, whereas transporters need to cycle through multiple states to translocate a single substrate ion24. Fig. 5: Schematic of ...
E. et al. Structure of human telomerase holoenzyme with bound telomeric DNA. Nature 593, 449–453 (2021). Article ADS CAS PubMed PubMed Central Google Scholar Nguyen, T. H. D. et al. Cryo-EM structure of substrate-bound human telomerase holoenzyme. Nature 557, 190–195 (2018). ...
Table S1. Analysis of previously communicated MS data with a focus on α-keto acid dehydrogenase complexes, related to Figure 2 Table S4. Quantification of clusters surrounding reference-free class averages of negatively stained single particles and embedded snapshot of 2D class averages, related ...