As one of the most successful therapeutic target families, G protein-coupled receptors (GPCRs) have experienced a transformation from random ligand screening to knowledge-driven drug design. We are eye-witnessing tremendous progresses made recently in the understanding of their structure–function relatio...
For large groups of cells, or for unlabeled single-cell data sets, we randomly subsampled the cells to reduce t-SNE runtime (when cells number in the thousands, it is nearly impossible to distinguish individual cells in the Structure plot anyhow). Even with this subsampling, the Structure ...
The CST–Polα/primase complex is essential for telomere maintenance and functions to counteract resection at double-strand breaks. We report a 4.6-Å resolution cryo-EM structure of human CST–Polα/primase, captured prior to catalysis in a recruitmen
Binding of oligonucleotides was characterized by changes in protein 15N HSQC signals, intensities, and chemical shift values as a function of the concentration of unlabeled nucleic acid. RNA binding assay RNA binding assays were carried out with 0.4 pmol of 32P 5′-labeled 14-mer polyrA and 21...
Different from the evaluation of DEG identification, cells in BONE_MARROW dataset were unsorted. Hence, we firstly identified cell types by mapping each cell in BONE_MARROW to a bulk cell type with the highest Spearman correlation [15]. Then, Monocle 2 were used to construct pseudo-temporal ...
Telomerase is a ribonucleoprotein (RNP) enzyme that extends telomeric repeats at eukaryotic chromosome ends to counterbalance telomere loss caused by incomplete genome replication. Human telomerase is comprised of two distinct functional lobes tethered b
L-Ergothioneine (ET), the 2-thioimidazole derivative of trimethylhistidine, is biosynthesized by select fungi and bacteria, notably Mycobacterium tuberculosis, and functions as a scavenger of reactive oxygen species. The extent to which ET broadly functi
(Fig.1a, bottom right; 3 animals, 1633 and 526 cells, respectively). Recording sites were localized to different cortical regions using a custom pipeline (Supplementary Fig.1a–eand “Methods”), which allowed us to triangulate the anatomical position of individual channels along each probe (...
It is constituted of a ~600 nm-long inner tube made of Hcp hexamers stacked on each other, and wrapped into a sheath-like structure20,21. The sheath-like structure is composed of rows of heterodimers of TssB and TssC (VipA and VipB in Vibrio cholerae), which assemble into an ...
Degenerative retinal diseases like age-related macular degeneration (AMD) are the leading cause of blindness. Cell transplantation showed promising therapeutic effect for such diseases, and embryonic stem cell (ESC) is one of the sources of such donor ce