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Abbreviations: Cryo-EM, Cryo-electron microscopy; ZIKV, Zika virus; NS1, non-structural protein 1; VH, variable region of immunoglobulin heavy chain; VL, variable region of immunoglobulin light chain; MAb, monoclonal antibodies. The outer polar surface of the NS1 dimer is exposed to the solvent...
(buffer: 25 mM Tris pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.01% LMNG and 0.001% CHS). A Zenix-C SEC-300 3-µm 300-Å 4.6 × 300-mm column (Sepax, 233300P-4630) at a flow rate of 0.35 ml min−1was used for the data shown in Extended Data Fig.1b. ...
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Immunoglobulin E (IgE) plays a pivotal role in allergic responses. The high-affinity IgE receptor, FcεRI, found on mast cells and basophils, is central to the effector functions of IgE. FcεRI is a tetrameric complex, comprising FcεRIα, FcεRIβ and a homodimer of FcRγ (...