Following a historical account on the establishment of the constitutional formula of sucrose and of its conformational features, the present possibilities for interactive graphics display of its molecular geometry, based on X-ray structural data, are given. In addition, the MOLCAD program-computed ...
The RsPSII-CAC sample collected from the sucrose density gradient was concentrated to 1.5 mg ml−1 (in chlorophyll) and placed into 90% (v/v) cold acetone. The mixture was centrifuged at 13,000 × g for 10 min to extract pigments. The supernatant (10 μl) was auto-...
π) for genes, using SNPs from 1135 Arabidopsis accessions [46]. The SNPs were masked for repeated sequences, and we required that at least half of the gene had sequencing coverage across the 1135 accessions to be included. We also excluded genes that overlapped...
For transformation, cells were incubated at 40 °C for 30 min with gentle agitation. Cells were then collected by centrifugation at 1,820 × g for 1 min, washed once with 10 mL TAP + 2% sucrose, and finally resuspended in the same buffer at a concentration of 2.0 ...
Vinegar is a naturally-occurring liquid that contains many chemicals, so you can't just write a simpleformulafor it. It is approximately 5-20% acetic acid in water. So, there are actually two main chemical formulas involved. Themolecular formulafor water is H2O. The structural formula for ac...
The crystals were then soaked in stabilization solution (2 M (NH4)2SO4, 0.1 M Tris-HCl, pH 8.0, and 20 mM MgCl2) with 0.1 mM Rif for at least 12 hr. The crystals were then prepared for cryo-crystallography by soaking in stabilization solution containing 50% (w/v) sucrose for 30 ...
acute barrier disruption (see above), fixed in 2% paraformaldehyde, 2% glutaraldehyde, 0.09 M potassium oxalate, containing 0.04 M sucrose overnight at 4°C, and then post-fixed in 1% OsO4, containing 2% potassium pyroantimonate, pH 7.4, for 2 h at 4°C in the dark (Menon et al, ...
Reporting rotation for different wavelengths enables multiple data point comparison with standards or other samples. The optical rotatory power due to a solute in solution may be specified by a statement of the type: α(589.3 nm, 20 °C, sucrose, 10 g dm−3 in H2O, 10 cm path) = +...
Cell pellets were resuspended in TES buffer (0.2 M Tris, 5 mM EDTA, 0.5 M sucrose, pH 8.0) equivalent to 5% of bacterial culture volume, and kept on a shaker at 4 °C for at least 60 min. Cells underwent osmotic shock by adding double the volume of TES diluted 1:4...
(5 g l−1), sucrose (103 g l−1), pH 7.5. The ISP2 agar plate consisted of glucose (4 g l−1), malt extract (10 g l−1), yeast extract (4 g l−1), pH 7.5, and agar (15.0 g l−1). The IPS2 agar plates were used for theE. ...