Expression and purification of Lit wild-type and mutant proteins for enzyme assays were performed as described above except that the buffer used for SEC was Buffer G (25 mM Tris/HCl, 80 mM NaCl, 10%(v/v) glycerol, 0.02%(w/v) LMNG, final pH 8.3). For selenomethionine (SeMet) lab...
We examined NRZ and LRZ gene sequence diversity compared to the chromosome arms. We calculated pairwise diversity (Pi,π) for genes, using SNPs from 1135 Arabidopsis accessions [46]. The SNPs were masked for repeated sequences, and we required that at least half of the gene had sequencing c...
The harvested cells were suspended in the lysis buffer (30 mM Tris-HCl, pH 7.5, 300 mM NaCl, 5% Glycerol) and disrupted by a high-pressure homogenizer (ATS Engineering Inc) at 900 bar for 4 cycles. For the purification of TEF14, the TALON metal affinity resin was used and the...
MFPCP was crystallized in 2–4 μL drops with a protein to reservoir ratio of 1:1 by the hanging-drop vapor diffusion method, using a reservoir with 14 % PEG8000, 10 mM Tris-HCl pH 7.5, 200 mM MgCl2. Crystals were harvested in nylon loops, briefly soaked in reservoir buffer supplement...
The general synthetic methods involve the reactions between the hal- ogen–phosphorus compounds with an amine either alone (in excess) or in the presence of another HCl scav- enger (such as triethylamine). The main challenge in these reactions is the separation of the phosphoramidate from the ...
A cis square-planar structure exists for 2 with monodentate pyridazines. [Cu(L2) 2 ](ClO 4 ) 2 CH 3 CNCHCl 3 (5) crystallized in the triclinic system, space group [Formula: see text] with a=12.888(4), b=17.462(6), c=10.906(1), α=96.07(2)°, β=104.18(2)°, γ=94.51(...
1. A pharmaceutical composition for treating a subject suffering from an androgen receptor associated medical condition, comprising: a compound according to formula VI: wherein: L is a carbonyl, alkylene, alkenylene, or alkynl when Z is not present; Z is —H, —OH, a substituted styrenyl, ...
1. A polyether of the formula: ##SPC2## 2. Polyether of claim 1 which is a reaction product of an unsubstituted, halogen-substituted or alkyl substituted hexahydrophthalic acid diglycidyl ester with a cycloaliphatic diol in a molar ratio of 1:0.8 to 0.8:1. 3. Polyether of claim 2 in...
Based on the mass (Figure S18) and 13C NMR spectra, it was determined that the peak of the molecular ion for compound AB-05 was located at 792 [M]+. This result was in accordance with the chemical formula of the acyl tetraglycoside, which is C32H56O2. It was determined that the ...
The protein was eluted with 25 mM Tris/HCl pH 9.3, 8% (v/v) 2-propanol and a final purification was performed by size exclusion chromatography in 10 mM sodium phosphate buffer pH 8 using a Superdex 75 10/300 GL column (GE Healthcare Biosciences). The recombinant protein was ...