coccineus sucrose 6F-phosphate phosphorylase at 35 ◦C and 50 mM 3-(N-morpholino)propanesulfonic acid (MOPS) pH 7.0 (phosphorolysis) or 50 mM MES pH 6.5 (synthesis). Reaction Phosphorolysis Synthesis Substrate sucrose 6F-phosphate phosphate α-d-glucose 1-phosphate fructose 6-phosphate KM (...
Briefly, semen was gradually diluted at 37 ◦C with tris-yolk fructose (TYF) extender containing 3.028 g/dL trisaminomethane, 1.675 g/dL citric acid anhydrous, 1.25 g/dL fructose, 7% (v/v) glycerol, 20% (v/v) egg yolk, 100 IU/mL penicillin, and 100 µg/mL streptomycin. The ...
Individual components of SS showed different responses to drought and waterlogging across tissues: glucose concentrations increased significantly with drought in all tissues but showed little response to waterlogging in twigs and stems; sucrose and fructose concentrations showed marked increases in leaves and...
In addition, the PPP also provides ribose 5-phosphate, as a precursor to nucleic acids, and several intermediate metabolites, such as fructose 6-phosphate and glyceraldehyde 3-phosphate [11]. The PPP has two phases: the oxidative and non-oxidative phase. In the oxidative phase, the first and...