Structural analysis of a-n-glucans by 13C-nuclear magnetic resonance, spin-lattice relaxation studies. Carbohydr. Res - Seymour, Knapp - 1980 () Citation Context ... site in several ways. The failure of CAL20 TEPC1035 to precipitate with glycogen is surprising. However, the average length ...
A set of spectroscopic, chemical and separation methods are used for this purpose. Among them, NMR spectroscopy is known as a powerful tool in structural analysis of glucans both in solution and in solid state. Along with chemolytic methods [methylation analysis (MA), periodate oxidation, ...
Structural analysis of soluble d-glucans from strains of streptococcus mutans by 13C-nuclear magnetic resonance spectrometry FR Seymour,RD Knapp,BL Lamberts 被引量: 0发表: 1980年 NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confusa Dextrans are the main...
Cyclic β-1,2-glucan synthase (CGS) is a key enzyme in production of cyclic β-1,2-glucans (CβGs) which are involved in bacterial infection or
The fundamental and assorted roles of β-1,3-glucans in nature are underpinned on diverse chemistry and molecular structures, demanding sophisticated and intricate enzymatic systems for their processing. In this work, the selectivity and modes of action of a glycoside hydrolase family active on β-...
Here we present a structural and functional analysis of the recently established CBM family 92. All proteins analysed bind preferentially to β−1,6-glucans. This contrasts with the diversity of predicted substrates among the enzymes attached to CBM92 domains. We present crystal structures for two...
and they often use CBMs to improve reaction rates and retention of released sugars. Here we present a structural and functional analysis of the recently established CBM family 92. All proteins analysed bind preferentially to β−1,6-glucans. This contrasts with the diversity of predicted substrate...
2.2. Structural analysis of transglycosylation products Five major glucosylation products corresponding to peaks F1-F5 (Fig. 1) were isolated from the incubation mixture of Gtf180-ΔN for structural analysis by MALDI-TOF-MS and 1D/2D 1H and 13C NMR spectroscopy. The purity and retention time ...
To verify that these intermolecular interactions occur in solution and with β-glucan, we performed a mutational analysis of PlodiaβGRP-N. A series of single/double mutants was prepared (H31A, L41A, R48A, D49A, W76A, Y78A, R87A, H31A/R48A, and R48A/R87A), focusing on the residu...
ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene. Glycobiology 9, 31–41 (1999). Article CAS PubMed Google Scholar Bi, Y., Hubbard, C., Purushotham, P. & Zimmer, J. Insights into the structure and function of membrane-integrated processive ...