不同DNA提取试剂盒提取作物种子基因组DNA效果的比较 热度: 一种快速、高质量、通用型基因组DNA提取试剂盒及DNA提取方法 热度: 相关推荐 StrawberryDNAExtraction AdaptedfromalabbyC.Sheldon Introduction: DNAisfoundincellsfromAnimalsandPlants.DNAisadoublestrandedmacromoleculecomposedof nucleotidebasespairingAdeninewith...
Module 3 : Strawberry DNA Extraction MaterialsAudience, Target
Another study investigated glycosidically bound aroma compounds and their odor qualities after an extraction process (Ubeda et al., 2012). Glycosylation of furaneol was also studied with gene targets suggested for modifying the amount of free furaneol in the fruit (Song et al., 2016). The vast...
The pellets were washed in 2 mL of 0.9% NaCl and resuspended in 1 ml of TSB + 15% glycerol for storage at -80 °C until DNA extraction. DNA extraction and microbiome sequencing Microbial pellets were processed using DNeasy® PowerSoil® Pro Kit (QIAGEN, Hilden, Germany), following...
Modified CTAB method for high quality genomic DNA extraction from medicinal plants Pak J Biol Sci, 14 (2011), pp. 998-999 CrossrefGoogle Scholar Bent, 2006 Bent A. Arabidopsis thaliana floral dip transformation method Methods Mol Biol, 343 (2006), pp. 87-103 View in ScopusGoogle Scholar Bh...
DNA extraction We extracted genomic DNA from young leaves of greenhouse-grown parents and progeny using a DNeasy Plant Maxi Kit (Qiagen Inc., Valencia, CA, USA). We quantified extracted DNA using the Spectramax 190 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA) and diluted it to 30...
DNA extraction and quantification DNA was extracted from young leaves of the parents 'H4' and 'FV', along with the F1 and 335 F2 seedlings of the progeny, using a modified version of the CTAB method.31 The DNA was quantified using a Nanodrop spectrophotometer (Thermo Fisher Scientific, ...
Total DNA/RNA was isolated from strawberry leaves and roots using a plant total DNA/RNA extraction kit (Tiangen, Beijing, China). First-strand cDNA was synthesized using the RevertAid First Strand cDNA Synthesis Kit and used as the template for RT–qPCR (Transgen Biotech, China). Primer inform...
DNA extraction and PCR analysis DNA from regenerated hairy roots of transgenic and non-transgenic roots of YW (MS suspension-infested hypocotyls as a negative control) was extracted using the cetyltrimethyl ammonium bromide (CTAB) method according to Oosumi et al. [8]. First, hairy roots were ...
Plasma was collected from the unfasted experimental mice after a 12-week treatment for metabolite analysis at two different time points (9 am and 9 pm). These two samples were pooled for each mouse. The extraction and analysis of plasma metabolites were performed as described previously and pro...