Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Res. 39, e142 (2011). Article CAS PubMed PubMed Central Google Scholar Kariko, K., Muramatsu, H., Keller, J. M. & ...
Although the Mw irradiation has the advantage of shortening reaction time, this procedure needs an excess of quinone that impeeds the purification of the final product. Several derivatives of cycloadducts 23 and 29 were evaluated in vitro for determinig their cytotoxicity against the human cell ...
The invention provides methods for screening compound or chemical libraries by analyzing expressed RNA samples from biological samples treated with members of a compound library in a high throughput format.Inventors: Monforte, Joseph (Kensington, CA, US) Application...
After the digestion, the mixture was centrifuged at 10,000 × g at 4 °C for 10 min. Then, the supernatant was purified from the concentrated mixture using Iodixanol Gradient Ultracentrifugation method (Addgene). After purification, a 100 kDa protein ultrafiltration tube was applied ...
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Na+ ions were added to neutralize the system, and the TIP3P water model was used to solvate each system, ensuring a solvent layer of at least 10 Å from any point on the protein surface. Charges and parameters for ligands were generated with the Antechamber module using the AM1-BCC ...
we did not exclude data from the meta-analysis because we based our analysis on changes from baseline, which might be more efficient and powerful than the comparison of post-intervention values solely according to the current version of the Cochrane Handbook for Systematic ...
protein, resulting in Ab-protein-DNA complex. By purifying this complex and precipitating it, DNA to which this protein binds is selected. Subsequently, the DNA can be used for library construction and sequencing. I.e., this is a method to perform a complexity reduction in a non-random ...
Danser AHJ, Schunkert H (2000) Renin‐angiotensin system gene polymorphisms: potential mechanisms for their association with cardiovascular disease. Eur J Pharmacol 410:303–316 PubMedCASGoogle Scholar Dash VB, Junius AMM (1987) A handbook of Ayurveda. Concept, New Delhi ...
Chip technology, nor is it intended to review the various applications other than protein expression profiling that can be performed using the technology; for these, the reader is referred to several reviews listed in the reference section(1–6)....