Handbook貌似没有ISBN也没有年份,那就标记到这本书上了,做这一行嘛,克隆、纯化、冻干都必须会。20200513: 目前通俗易懂,逻辑简单,很多是经验之谈,书有点厚。 > 更多短评 1 条 我要写书评 Strategies for Protein Purification and Characterization的书评 ··· ( 全部0 条 ) 论坛 ··· 在这本书的...
After protein expression for each cultivation, bacterial cells were collected by centrifugation at 3,500gfor 20 min, and the resulting cell pellet was resuspended in phosphate-buffered saline (PBS). For cell disruption, the suspension was sonicated for five cycles of 1 min with an interval of 1...
Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Res. 39, e142 (2011). Article CAS PubMed PubMed Central Google Scholar Kariko, K., Muramatsu, H., Keller, J. M. & ...
Bertrand L, Parent S, Caron M, Legault M, Joly E, Angers S, Bouvier M, Houle B, Ménard L (2002) The BRET2/arrestin assay in stable recombinant cells: a platform screen for compounds that interact with G protein‐coupled receptors. J Receptor Signal Transduct Res 22:533–541 CASGoogle ...
Chip technology, nor is it intended to review the various applications other than protein expression profiling that can be performed using the technology; for these, the reader is referred to several reviews listed in the reference section(1–6)....
After the digestion, the mixture was centrifuged at 10,000 × g at 4 °C for 10 min. Then, the supernatant was purified from the concentrated mixture using Iodixanol Gradient Ultracentrifugation method (Addgene). After purification, a 100 kDa protein ultrafiltration tube was applied ...
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we did not exclude data from the meta-analysis because we based our analysis on changes from baseline, which might be more efficient and powerful than the comparison of post-intervention values solely according to the current version of the Cochrane Handbook for Systematic Re...
[51]. For a protein to be considered a hit a minimum coverage of 50% for its whole length as well as the HMM model was mandatory, using ane-value threshold of 1e−3. Protein domains were annotated using InterProScan [52] with default parameters. Metabolic pathways were inferred from ...
Granell, A., M. Cercos & J. Carbonell.1998. Plant cysteine proteinases in germination and senescence. Pp 578–583.In:A. J. Barrett, N. D. Rawlings, & J. F. Woessner (eds). The Handbook of Proteolytic Enzymes. London Academic Press, San Diego. ...