The Rep proteins of ssDNA viruses of prokaryotes (bacteria and archaea) and eukaryotes display distinct domain organizations2. In eukaryotic CRESS-DNA viruses, the endonuclease domain is fused to a superfamily 3 helicase (S3H) domain22, which is responsible for unwinding of the double-stranded (ds...
The conformational space sampled by P8 trimers in solution and at different Ca2 þ concentrations remains unknown. Insolubility of purified P8 and its tendency to readily form large assemblies even in the presence of Ca2 þ -chelating agents has impeded its solution structure studies by small ...
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