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Live cell imaging demonstrated persistent associations between fluorescence-labelled HaloTag-ZNF827 and both GFP RPA32 (Supplemental Movie 1) and PCNA chromobodies (Supplemental Movie 2) in U-2 OS cells. Co-IP of ZNF827 identified an interaction with both ATRIP, the regulatory partner of ATR, ...
Supplementary movie (MOV 4492 kb) Rights and permissions Reprints and permissions About this article Cite this article Ackermann, D., Schmidt, T., Hannam, J. et al. A double-stranded DNA rotaxane. Nature Nanotech 5, 436–442 (2010). https://doi.org/10.1038/nnano.2010.65 Download citation...
Read the full-text online article and more details about "Video: FedEx Male Goes Adrift; Tom Hanks Excels in This Lengthy Movie about Being Stranded on a Desert Island, but the Flawed Storyline Is All at Sea" - The Mirror (London, England), July 13, 2001...
摘要: Read the full-text online article and more details about "Video: FedEx Male Goes Adrift; Tom Hanks Excels in This Lengthy Movie about Being Stranded on a Desert Island, but the Flawed Storyline Is All at Sea" - The Mirror (London, England), July 13, 2001...
The large dataset of wild-type TLR3 with 90 bp dsRNA collected from CRYO ARM 300 microscope (Supplementary Fig.1) was processed using cryoSPARC28. 12,825 raw movie stacks were motion-corrected using the patch motion correction, and the CTF parameters were determined using the patch CTF estim...
4 and Supplementary Movie 1. the NC, we hypothesize that Ca2 þ induces transition of the P8 trimer from the closed to the open conformation and this further facilitates outer shell assembly by domain swapping (Fig. 5d,e). Due to steric clashes, the P8 monomers closest to the P4 ...
Full size image Last but not least, Ni-IRMOF-74-II was used in gene silencing to further confirm its potential for intracellular delivery and biological applications. DNAzyme (a 33-nucleotide ssDNA) was particularly designed with sequence in complementary to part of target EGR-1 mRNA, which gen...
A fluorescence movie showing translocation of UvrD on a long ssDNA in real time. The ssDNA molecule is not visible but laid vertically in the center of the movie, with its 3′-end at the top and its 5′-end at the bottom. Raw data from an EMCCD camera (2 bytes per pixel) were con...
Full size image An association between DBD-A and DBD-E In order to confirm the specific DBD-A–DBD-E interactions at the dimer interface, we quantified these interactions between purified domains using microscale thermophoresis (MST). We produced recombinant DBD-A (Supplementary Fig9) and DBD-E...