A general method and strains of bacteria are described, by means of which it is possible to dramatically purify plasmid DNA with respect to genomic DNA, and RNA. In one preferred embodiment, lysis and nuclease removal of host nucleic acids is an integral component of the fermentation/harvest ...
DescriptionAlthough most strains of E. coli bacteria are harmless and live in the intestines of healthy humans and animals, several strains can produ...doi:10.1002/chin.200127039cyclopropanation reactionsdiastereoselective syntheses, enantioselective syntheses (incl. ci/trans‐isomerism...
The capacity of 17 strains of E. coli (12 of them - uropathogenic, 4 - from other pathotypes, and one non-pathogenic) to form biofilms was examined. The bacteria were cultivated on microtitre plates on minimal glucose medium M63 for 48 h, the attached cells were stained with crystal viole...
Evolution of knockout (KO) strains from a pre-evolved (i.e., optimized) wild-type strain.aExperimental design using adaptive laboratory evolution (ALE) and enzyme knockouts to investigate system re-optimization following major metabolic perturbations.bAn isolated wild-type (wt)E. coli(MG1655 K-1...
[Typing of E. coli strains isolated from a turkey and a test of their sensitivity in relation to antibiotics] 来自 NCBI 喜欢 0 阅读量: 29 作者: M Minchev 摘要: The turkey-poults on an industrial turkey-breeding farm were studied. A total of 75 strains of Escherichia coli were isolated....
coli strains is by IPTG7,8. IPTG is not metabolized by bac- teria, which is why one-point addition is sufficient to guarantee induction5. However, IPTG is known to put a high metabolic burden on E. coli9,10 and is often associated with the generation of misfolded protein aggregates, ...
E Karisik 被引量: 0发表: 2007年 Molecular characterization of multi-drug resistant Escherichia coli isolated from urine samples in Mater Hospital, Nairobi, Kenya Multi-drug resistance among Gram negative bacteria is on the increase due to acquisition and expression of extended spectrum 尾-lactamases ...
Microbiological culture, isolation and identification of bacteria followed. Bacteriocin production was assessed by growth inhibition of indicator strains E. coli K12-Row, E. coli C6 (phi), and Shigella sonnei 17. Identification of bacteriocin-encoding determinants and E. coli phylogroups was performed...
Together, these findings suggest that the colonic E. coli strain variation and abundance act to provide a functional complex that interacts with host metabolism and immunity. Thus, understanding the response of the host colonic cells to a single strain of bacteria SCIENTIFIC REPORTS | 3 : 3416 |...
Bacteria from the Turicibacter genus are prominent members of the mammalian gut microbiota and correlate with alterations in dietary fat and body weight, but the specific connections between these symbionts and host physiology are poorly understood. To a