RESULTS: Live neuron staining with BODIPY FL-X thapsigargin (Invitrogen, Carlsbad, CA) revealed a 40% decrease in sarco-endoplasmic reticulum Ca-ATPase binding in axotomized L5 neurons and a 34% decrease in L4 neurons. Immunocytochemical labeling for the ER Ca-binding protein calreticulin was ...
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Gene expression was assessed by quantitative real-time polymerase chain reaction, using Applied Biosystems Taqman gene expression assay probes for fpn, transferrin receptor 1 (tfr1), (divalent metal transporter 1) dmt1, hamp, and house-keeping gene β-Actin (Life Technologies, Carlsbad, CA). The...
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