Stopped-flow FRET is a usefultechnique for studying many types of biomolecular interactions. The method provides kineticinformation regarding distance changes occurring on the millisecond timescale. This article gives an introduction to the technique along with a review of some example experiments from ...
Stopped-flow FRET measurements.Barbara MüllerMaria AndersJochen Reinstein
Chirascan 系列圆色光谱仪可以扩展到动力学停流stopped flow模式, 实现动态(时间维度下) 的光属性检测(圆色CD,吸收 absorbance , 荧光 fluorescense , 荧光偏振 FP , 双路荧光DF, FRET 等) 分析, 研究快速反应(ms )的动力学。 Chirascan 系列圆色CD光谱仪可以升为全自动通量HTS (High Throughput) 模式, 实现...
More sophisticated experiments such as Fluorescence resonance energy transfer (FRET) measurements are also possible with an LED light source.[6] A key advantage at a number of wavelengths is the combination of very high light intensity and stability, which work together to dramatically reduce signal...
FRET detecti on) 8. Ni jvipakul, S., Ballou, D.P., Chaiyen, P. (2010) Reduction Ki netics of a Flavi n Oxidoreductase LuxG from Photobacterium leiognathi (TH1):Half Si tes Reacti vi ty. Biochemi stry, 49(43), pp. 9241-9248. 9. Gahl, R.F., Pradeep, L., Siegel, ...
Imaging of PKC activity and DG production in real time in live cells with FRET-based reporters has revealed that PKC activity is localized to those cellular locations where DG is generated (7, 8). Thus, PKC has served as a paradigm for an enzyme that is reversibly activated by membrane ...
As the most sensitive fluorescent markers of the enzymatic process, we have chosen Trp and aPu residues and the FRET pair FAM/BHQ1. When fluorescently labeled substrates have been tested, it has been found that the bending of the DNA duplex and its local melting occur in the initial complex...
correlation between FRET assay results and theMM-GBSAcalculated binding energy (Table 2) had been explored in Figure 4, and the correlation coefficient was 0.75. 2.6. Competition Dialysis Assay. Competition dialysis assay was used to evaluate the selectivity of 5-N-methyl quindoline ...
Collisional transfer, diffusional transfer, cholesterol, cholesterol trafficking, FRET, stopped flow, fluorescence, model membranes, small unilamellar vesiclesIn this chapter we describe the use of stopped flow fluorescence spectroscopy to analyze the kinetic mechanisms of protein mediated cholesterol transfer ...
We have developed and optimized a stopped-flow fluorescence assay for use in studying DNA unwinding catalyzed by Escherichia coli RecBCD helicase. This assay monitors changes in fluorescence resonance energy transfer (FRET) between a pair of fluorescent probes (Cy3 donor and Cy5 acceptor) placed on...