网络终止密码子通读 网络释义 1. 终止密码子通读 因此,可以使用该技术研究程序性框移(programmed frameshift)和终止密码子通读(stop-codon readthrough)等现象。Wei… 5527lok.blog.163.com|基于17个网页
Stop codon readthrough is essential to diverse viruses, and phylogenetically predicted to occur in a few hundred genes in but the importance of regulated readthrough in eukaryotes remains largely unexplored. Here, we present a ribosome profiling assay (deep sequencing of ribosome-protected mRNA ...
Stop codonreadthrough is a common strategy of viruses to encode proteins with an extendedC terminus[84]. For instance, a ~5% probability of readthrough of thegagUAG stop codon is required for producing the Gag-Pol polyprotein necessary for the assembly of themurine leukemia virus[85]. Stop ...
We further demonstrate that readthrough occurs in yeast and humans. Readthrough thus provides general mechanisms both to regulate gene expression and function, and to add plasticity to the proteome during evolution. 展开 关键词: Molecular biology Genetics Stop Codon Readthrough is a Feature of ...
We aimed to investigate the use of stop codon read-through drugs in a genomic reporter assay cell line with a defect in the mutase gene. A single C-T base change was introduced into exon 6 of the human MUT sequence in the BAC clone RP11-463L20 resulting in an arginine residue being ...
encoding a cell membrane anchoring peptide, a reporter peptide or an epitope tag downstream of the stop codon, and where the cells are cultured in the presence of a termination suppression agent, in particular an aminoglycoside antibiotic, that results in regulated stop codon readthrough. ...
The approach consists of introducing a premature termination codon (PTC) in the Cas9 gene and subsequently treating with an aminoglycoside drug, which allows readthrough of the complete protein. To validate that system, HEK293T cells were co-transfected with a PX458 plasmid, which was mutated to...
Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the function or mechanism of readthrough. If an mRNA...
(UGA) stop codons are read through; there are no examples of read through of the natural ochre (UAA) stop codon. However, when the suppressible UAG codon of TMV is replaced by a UAA codon the virus can still replicate and produce mature virions (Ishikawaet al., 1986). Unlike with...
CRISPR-SCReT (CRISPR-Stop Codon Read Through) method to control Cas9 expression for gene editing 来自 Nature 喜欢 0 阅读量: 61 作者:P Yaméogo,BL Duchêne,N Majeau,JP Tremblay 摘要: CRISPR/Cas9 has paved the way for the development of therapies that correct genetic mutations. However, ...