This study examines the validity of measuring faecal bile acids (FBA) in a single stool sample as a diagnostic tool for bile acid diarrhoea (BAD) by direct comparison to thedoi:10.1038/s41598-022-12003-zAditi.KumarThe Royal Wolverhampton NHS Trust, Wolverhampton Road, Wolverhampton, WV10 0QP...
Study approval was granted by NHS Lothian SAHSC Bioresource (SR211), with ethical approval to test anonymised, excess patient samples (10/S1402/33). Competing interests The institution conducting this research received free consumables and reagents from Becton Dickinson and Cepheid Inc. The research...
Samples should never be re-frozen, because freeze-thaw cycles may compromise stability and efficacy of the sample, possibly because of loss of viability. To eradicate vegetative cells of C. difficile, prior to FMT (until 1 day before the procedure), patients receive vancomycin (125–250 mg ...
To determine the limit of detection (LoD) for the detection of HAV by RT-PCR, we spiked pooled normal human serum (NHS) with known concentrations of a plasmid containing the 5′-untranslated region (5′-UTR) of HAV (pUC57-5′-UTR-HAV). We determined that the LoD of the RT-PCR ass...