The GI Pathogen Profile, multiplex PCR provides assessment of 22 specific gastrointestinal pathogenic microbes using FDA-cleared, real-time PCR. The Centers for Disease Control (CDC) estimates that 350 million acute diarrheal illnesses occur annually, and notes that diarrheal syndromes are often similar...
E. faecalis isolates have been recognized as the second uropathogen in some countries [2, 3]. CA-UTI is a public health threat [6]; it can be mainly caused by E. coli and Klebsiella spp.; however, other bacteria, such as Enterococcus, Proteus, Pseudomonas aeruginosa, and Staphylococcus ...
The positive control of the sample pool was included in the gel processing steps, and no template control of PCR grade water used to screen out potential laboratory contamination. The isolated DNA template were measured for their quality in purity ratio and concentration using the NanoDrop OneC spe...
In particular, DNA extraction and PCR protocols vary substantially among reference laboratories. These differences can have a substantial impact on the analytical sensitivity and specificity of bacterial culture and PCR assays. Culture-dependent methods should be employed if a specific pathogen is ...
Molecular diagnostic assays such as PCR do not rely on phenotypic traits, but rather screen for the genetic determinants that define STEC. As a result of this broad genotypic screen, patients' stool samples containing non-O157 STEC can be readily identified that otherwise would have gone ...
Human Rotavirus (HRV) is the causative pathogen of severe acute enteric infections that cause mortality among children worldwide. This study focuses on developing a new and effective treatment for rotavirus infection using an extract from Saccharomyces cerevisiae, aiming to make this treatment easily ac...
diarrhea, PCR, military, infectious diseaseWe evaluated stool enteropathogen detection by semiquantitative polymerase chain reaction (PCR) in 108 subjects with travelers’ diarrhea before and 3 weeks after treatment. Stool samples from 21 subjects were positive for the same pathogen species at both ...
Combined stool-based multiplex PCR and microscopy for enhanced pathogen detection in patients with persistent diarrhoea and asymptomatic controls from Coite d'Ivoire. Clin Microbiol Infect 2015;21. https://doi.org/10.1016/j. cmi.2015.02.016.Becker, S.L.; Chatigre, J.K.; Gohou, J.P.; ...
Compared to the traditional polymerase chain reaction (PCR) technique, QLAMP-PhR offers several advantages including rapidity, simplicity, specificity, sensitivity, and cost-effectiveness method that can quantitatively screen for Fn presence in normal populations. The QLAMP-PhR method represents a ...
doi:10.1093/ofid/ofz187THERAPEUTICSCRYPTOSPORIDIUMDIARRHEAMILITARY personnelTisdale, Michele DTribble, David RTelu, KalyaniFraser, Jamie AConnor, PatrickPhilip, CliffOdundo, ElizabethReyes, FaviolaSimons, Mark PSwierczewski, BrettOpen Forum Infectious Diseases...