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Users can specify a range of memory to monitor and anytime the program writes to that range, a snapshot will be taken and stored to disk. This makes it very straightforward to monitor a static region of memory for changes. Pointy Stick is a tool that allows tracing of program execution ...
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The cell suspension was snap frozen in liquid nitrogen. Samples were agitated using a tube rotator at 4°C for 15 minutes followed by incubation at −80°C overnight. Samples were then centrifuged at 15,000 rpm, 4°C for 10 minutes. The supernatants were collected and stored in ...
Functional consequences of nsSNPs in the coding region were predicted by using SIFT, PolyPhen 2, I-Mutant 3.0, PROVEAN, P-Mut, SNAP2, PON-P, and Mutation Assessor algorithms. SIFT uses the homology sequence of the proteins and alignment of natural nsSNPs with orthologous and paralogous protein...
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DNA was eluted from the membranes by incubation at 95°C for 2 min, mixed briefly by vortexing, and then snap-frozen in dry ice. The samples were allowed to thaw slowly on ice and mixed. DNA eluted from the membranes was recovered by alcohol precipitation, resuspended in 50 µl of ...
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Under harmonic force input, data was obtained only at 0.1 Hz and 0.5 Hz, as the testing machine could not move at the high-speeds associated with VMT snap-through. The energy dissipated (hysteresis loop areas) in these tests was slightly lower than that in the displacement controlled tests ...