www.nature.com/scientificreports OPEN received: 25 September 2015 accepted: 18 January 2016 Published: 16 February 2016 Mechanical stretching stimulates collagen synthesis via down- regulating SO2/AAT1 pathway Jia Liu1, Wen Yu1,Yan Liu2, Selena Chen3,Yaqian Huang1, Xiaohui Li4,...
The membrane was then blocked in Tris‐buffered saline (pH 7.5) containing 0.1 % Tween‐20 (TBST) and 5% non‐fat dry milk for 1 h at room temperature. Next, they were incubated overnight in primary antibodies at a 1:500 to 1:2000 dilution. The primary antibodies were directed against...
acirc_104075 was overexpressed or knocked down with the indicated plasmids, and circ_104075 expression was measured in Bel-7402 and SMMC-7721 cells using qPCR.b,ccirc_104075 was overexpressed or knocked down with the indicated plasmids, and the mRNA level of the indicated HCC stimulators was me...
On the next day, the membrane was washed with PBS-T three times and incubated with appropriate secondary HRP antibodies in 5% milk at room temperature for 1 h. The membrane was washed again with PBS-T three times and ECL was applied for film development. Protein immunoprecipitation (IP) ...
Membranes were blocked in 5% milk in TBST (50 mM Tris pH 7.4, 150 mM NaCl, 0.1% Tween 20) and incubated overnight at 4°C with the indicated primary antibodies followed by incubation with horseradish peroxidase (HRP)-tagged anti-rabbit or anti-mouse secondary antibodies for one h. Protein...
However, chemerin (100 ng/mL) stimulated BME-UV1 cells to synthesize and secrete αS1-casein, the major protein component of milk. These results indicate that chemerin may be a potent regulator of the bovine mammary epithelial cells' functional differentiation, contributing, along with the major ...
Regardless of whether the cells were treated with rapamycin or XAV939 alone or in combination, both mTORC1 and Wnt/β-catenin pathways were down-regulated, accompanied by a decrease in CDX2 expression. Taken together, our data indicate that CDX2 stimulates porcine intestinal epithelial cell ...