The aggregate was covered with a sealing layer of Matrigel mix and incubated at 37°C for polymerization after which DMEM with 10% FCS and pen/strep supplements was added. The extent of outgrowth was followed for 5-6 days, and media were changed to 0% FCS conditions 24-48 h prior to ...
3.1. Isolation and Characterization of St. John’s Wort Herb Polysaccharides Carbohydrates were isolated from defatted St. John’s Wort herb in a yield of 1.3% related to the starting material. The ochre brown product was assessed to be a polysaccharide with neutral sugar content of 78%, 16%...