We observed that a microsomal protein which depends on ATP hydrolysis is involved in membrane insertion of both, ribonucleoparticle-dependent and -independent precursor proteins. The azido-ATP sensitive protein was shown to be distinct from the lumenal (termed trans-acting) molecular chaperone BiP....
S.K.B was involved in all aspects of the work including single-molecule assay development performing experiments and data analysis. S.K.B. and M.J.L. designed the experiments and prepared the manuscript. S.S. expressed and purified the isolated domains and carried out the Biacore-binding stu...
the use of the mouse tyrosine hydroxylase limits its production. Here, we design an efficient whole-cell catalyst of hydroxytyrosol inEscherichia coliby de-bottlenecking two rate-limiting enzymatic steps. First, we replace the mouse tyrosine hydroxylase by an engineered...
The FS has been proposed to function as a protective girdle for the axoneme [1, 2], influence the degree of flexibility, plane of flagellar motion, the shape of the flagellar beat and as a scaffold for enzymes involved in signal transduction, including protein kinase A by anchoring to AKA...
the morphogenesis of precartilage condensations [2, 5], the spatiotemporal patterning of the condensations [3, 43], and the specification of the identity of each skeletal element [44–46], we here demonstrate that CG-1A and CG-8 (two of the five CGs) are involved in at least the firs...
Molecular docking of C-A1 (in yellow) in HIV-1 hexameric capsid structure 4XFZ.pdb with bound PF-74 (in green) using the Compute: SiteFinder option in MOE software. A single C-A1 conformation is shown bound to capsid. The two subunits of capsid are depicted inbrownandturquoiserespectively...
It has been reported [55] that a specific amino acid within STIM1 α2 (F394) is involved in the activation of Orai1 channels. Therefore, it was proposed that the α2 region acts as a putative gating/coupling site. Substitution by a histidine at this position (F394H) resulted in impai...
the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy,we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking.FENG...
The combined crosslinking results therefore provide strong evidence for a coalescence of the three TatABC subunits in the trans-sided half of the bilayer with the N-termini of TatA and TatB possibly being involved in the association of the three Tat subunits. RR-signal peptides contact oligomers...
Exocyst is a large multisubunit tethering complex essential for targeting and fusion of secretory vesicles in eukaryotic cells. Although the assembled exocyst complex has been proposed to tether vesicles to the plasma membrane and activate soluble N-ethy