RT-PCR Determine which RT-PCR procedure to use In performing RT-PCR, one-step and two-step methods are the two common approaches, each with its own advantages and disadvantages. One-step RT-PCR combines first-strand cDNA synthesis (RT) and subsequent PCR in a singl...
PCR amplifies a target DNA sequence, making many copies of that sequence. Why is it important to perform PCR before gel electrophoresis? Explain the importance of performing PCR as it relates to gel electrophoresis. Describe three ways that PCR is similar to DNA replication in cells...
Denaturants help maintain RNA in single-stranded form, allowing more predictable sample migration and separation results. When performing RNA gel electrophoresis, DNA ladders should be avoided, because their use under denaturing conditions can...
By performing PCR with a single SINE primer in combination with a panel of arbitrarily selected unique primers, we have demonstrated that, as in human, polymorphisms can be detected and typed in pig family DNAs. A large number of SINE primer x unique primer combinations have been screened ...
What are all the steps in performing a gram stain? What is the most crucial step? What is the 2 step process used to diagnose UTI? Explain how this serological test is used: Immunoblot procedures What are the more accurate sampling techniques used in ecology? What are some tools that you...
findings23. However, we cannot exclude the possibility that an oxidase might be responsible for enzymatic oxidation of R-SV into R-S in vivo. Taken together, our results suggest that Rif16, rather than performing a normal bio-oxidation, may catalyze an atypical P450 reaction in rifamycin ...
Expression plasmid with allmupgenes needed for conversion of PA-B to PA-A. (A) Plasmid map. (B) Functional bioassays for each gene in the constructed plasmids (see Table1) usingB. subtilis; the best performing IPTG induction condition is shown, which was 0.5 mM IPTG for ΔmupM[pJC...
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What are all the steps in performing a gram stain? What is the most crucial step? How might the bones tell an expert that person is an elderly of 70 years? Explain the condition of Pneumothorax and how it can be treated. How do drugs for alleviating osteoporosis ...
Classical microbial culture is still considered the gold standard in medical microbiology. Several molecular detection techniques have been implemented but these are generally geared towards specific pathogens (e.g. specific RT-PCR or microarrays). Even when unbiased molecular approaches are used, such ...