RT-PCR Determine which RT-PCR procedure to use In performing RT-PCR, one-step and two-step methods are the two common approaches, each with its own advantages and disadvantages. One-step RT-PCR combines first-strand cDNA synthesis (RT) and subsequent PCR in a single ...
The different types of PCR After performing a standard PCR reaction, you can determine the concentration, yield and purity of the amplified DNA sequences using gel electrophoresis, spectrophotometry or fluorometry. However, you can’t determine the amount of template DNA present in a sample before ...
What are all the steps in performing a gram stain? What is the most crucial step? What is the 2 step process used to diagnose UTI? Explain how this serological test is used: Immunoblot procedures What are the more accurate sampling techniques used in ecology? What are some tools that you ...
RNA laddersare usually provided with a loading buffer containing a denaturant. Denaturants help maintain RNA in single-stranded form, allowing morepredictable sample migrationand separation results. When performing RNA gel electrophoresis, DN...
Classical microbial culture is still considered the gold standard in medical microbiology. Several molecular detection techniques have been implemented but these are generally geared towards specific pathogens (e.g. specific RT-PCR or microarrays). Even when unbiased molecular approaches are used, such ...
findings23. However, we cannot exclude the possibility that an oxidase might be responsible for enzymatic oxidation of R-SV into R-S in vivo. Taken together, our results suggest that Rif16, rather than performing a normal bio-oxidation, may catalyze an atypical P450 reaction in rifamycin ...
It has the potential to be applied to situations in which a highly sensitive diagnosis is required. The newly developed SHIMADZU assay enables performing real-time RT-PCR without RNA purification, thereby substantially shortening the measurement time. This technology, called Ampdirect Technology [8],...
The article features Eugene Gifford Grace who from his position as resigned as chief executive of Bethlehem Steel Corp., seven months after he suffered a stroke. It states that Grace had made the company to be the second-largest steel producer in the U.S. and a shipbuilder during the World...
Expression plasmid with allmupgenes needed for conversion of PA-B to PA-A. (A) Plasmid map. (B) Functional bioassays for each gene in the constructed plasmids (see Table1) usingB. subtilis; the best performing IPTG induction condition is shown, which was 0.5 mM IPTG for ΔmupM[pJC...
After PCR, the amine-functionalized end of the tethers for isolated CD were crosslinked to ½ anti-biotin antibody (described later) while tethers for isolated CBM were crosslinked to anti-His antibody (Genscript—A000186-100), using EDC chemistry. Similar DNA linkers have been used in the ...