Our aim was to build two complete Asc-1 homology models of the states, “outward-open” and “outward-open occluded” conformations. Given the lack of X-ray crystal structures involving Asc-1 in OO and OOO states, homology models were derived from the well-resolved multi-states of the rela...
Using the new benchmark dataset, we proposed a new predictor called "dRHP-GreyFun" based on the grey modeling and functional domain approach. Rigorous cross-validations have indicated that the new predictor is superior to its counterparts in both enhancing success rates and reducing computational ...
Based on homology to defined proteins, a region shared between SWI1/DYAD1 and AM1 is predicted to have DNA binding ability like other PHD finger homeodomain proteins [10] and likely to be involved in regulating transcription. Our array data clearly demonstrate that AM1 attenuates the expression...
(4.1 protein, ezrin, radixin and moesin homology domain). A common domain found in a number of proteins that mediate linkage of the cytoskeleton to the plasma membrane. The FERM domain often interacts with the cytoplasmic tail of transmembrane proteins. Pleckstrin homology domain A lipid-binding ...
Structure of Plasmodium vivax dihydrofolate reductase determined by homology modeling and molecular dynamics refinement The structure of The structure of Plasmodium vivax dihydrofolate reductase, a potential target for antimalarial therapy, is described. G Rastelli,S Pacchioni,MD Parenti - 《Bioorganic & Med...
Using persistent homology to guide optimization has emerged as a novel application of topological data analysis. Existing methods treat persistence calculation as a black box and backpropagate gradients only onto the simplices involved in particular pairs. We show how the cycles and chains used in th...
The human mitochondrial AAA+ protein LONP1 is a critical quality control protease involved in regulating diverse aspects of mitochondrial biology including proteostasis, electron transport chain activity, and mitochondrial transcription. As such, genetic or aging-associated imbalances in LONP1 activity are ...
Using in vitro experiments, we reconstitute the key biosynthetic steps of linker formation and shuttling via CysB to the NRPS. The biosynthetic logic involves a previously uncharacterized bifunctional domain found in the stand-alone NRPS module CysH, albicidin biosynthesis and numerous BGCs of unknown...