For time-course experiments reactions were stopped either by addition of 25 mM EDTA (pH 8; ARH3) or 100 μM PDD00017273 (PARG; Tocris). Enzymatic activity analysis using luminescence detection of ADPr ARH3 mutant analysis was performed essentially as described in ref. 36. Briefly, 20...
Fading is where the builder uses a clipper and blades to work from long to super short progressively. If you want your long locks to be upgraded gradually, you could get a fade. It is trendier looking, with its edge and various variants, such as undercuts, customized plants, and line w...
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Probably one of the more tedious parts (Just from the sheer number of pieces, it was actually pretty simple to make) have got to be the gauntlets. They look great but are time consuming to make. They will however limit your movement in your hands so if you plan on using them for some...
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Child's Toy Light Switch Box: The inspiration for this project came from babysitting my 1-year old nephew. He will quickly lose interest in a room full of toys, but he LOVES light switches. The problem with light switches is that they are several feet of
Generally this value is kept lower than the length of the handlebar (max 600mm). In this way is simpler for the driver to understand where is possible to pass through and where not. We have therefore chosen a value of 530 mm, realized using three equally spaced tubes. ...
for the tank. This was left to cure for a couple of days. Finally, I hand painted the internals using automotive grade black paint to ensure gasoline resistance. Before the paint was able to dry, I carefully used a solvent (Eastwood Pre) to clean up any paint that had gone out of ...
World's Smallest Survival Kit V2.0: Meet the world's smallest survival kit V2.0! If you have not had a chance to check out the original follow this link:V1.0 This version has been fully submerged in water for over an hour, frozen into a block of ice, and
I lined up the mounting hardware on the wrist rest with my freshly drilled holes and screwed the two together using the male side of the Chicago screws. And just like that, we were done at Builders Anonymous. I thanked Robin and Ian for opening their doors to me and assisting me in thi...