Recommendations are provided for optimizing the immunization response.doi:10.1093/ilar.46.3.269Leenaars MarliesHendriksen Coenraad F. M.ILAR JIlar JournalLeenaars, M. and Hendriksen, C.F. 2005. Critical steps in the production of polyclonal and monoclonal antibodies: evaluation and recommendations. ...
Antibodies used are listed in Supplementary Methods. Cell cycle analysis Cells were centrifuged at 300 × gfor 5 min and stained in 500 μl of 20 μg/ml Hoechst 33342 (Biolegend) for 45 min at 37 °C. Cells were then centrifuged at 300 × gfor 5 min at 4 ...
“We buy our antibodies based on price alone.” These sections of human tonsil from the same block have been stained with the B cell marker CD20 using primary monoclonal antibodies from different sources (suppliers). In each case, the recommended pretreatment and optimized dilution were u...
Methods for altering the cellular secretion rate of a protein, such as an antibody and the altered cells produced by the method are disclosed. The methods and altered cells are useful for producing high levels of proteins for therapeutic, diagnostic or research purposes. 图片...
SR-BI antibodies and BLTs), which could be interpreted as a higher SR-BI dependence for entry. Thus, these results indicated a role for E2/SR-BI interaction for the entry process at a post-binding step. Consistently, as compared with the HVR1-mutated viruses, we found that wt HCVcc di...
The tubulin epitope recognized by polyreactive IgG1Per was similar to that of germ-line-encoded polyreactive antibodies. It is hypothesized that IgG4Per- and IgA1Per-producing cells derive from the IgG1Per polyreactive clone after somatic events leading to the production of monoreactive antibodies...
A method for the production of hybridoma cell lines producing monoclonal antibodies capable to specifically binding to a human C44-fragment of agrin A method for the production of a hybridoma cell lines producing monoclonal antibodies capable to specifically binding to a human C44-fragment of agrin...
The large scale production of monoclonal antibodies presents a challenge to design efficient and cost effective downstream purification processes. We explored a two stage resin screening approach to identify the best candidates to be utilized for the platform purification of monoclonal antibodies. The ...
In this study, virus elimination by the chromatographic steps used during the purification process for two (IgG-1 & -3) monoclonal antibodies (MAbs) have been investigated. Both the Protein G (>7log) and ion-exchange (5 log) chromatography steps were very effective for eliminating both ...
Analysis by Monoclonal Antibodies of Cell Surface Structures Marking Discrete Steps of Cell Differentiationdoi:10.1016/B978-0-08-033215-4.50156-4A. FunaroG. BelloneS. DemariaM. ZaccoloC. TettaS. RoggeroF. GenzanoF. Caligaris‐cappioA. Cappa...