However, the commonly used protocols are usually costly and time consuming. Hence, this study developed a four‐primers‐based miRNA quantification method, and simultaneously performed both reverse transcription and PCR by a one‐step operation on a real‐time system. Moreover, we designed the ...
ABI Step One Plus PCR仪全貌如图1,需搭配Step One Software软件使用(工程师会预设软件在电脑上)。 图1 ABI Step One Plus仪器 图2 仪器开机页面 二、使用方法 注意: 本操作指南仅针对SYRB染料法的操作,且本仪器必须使用含ROX的qPCR试液,推荐使用含High Rox的染料。 机器反应舱标配pcr管为100μl,通常配制试剂...
4、One-step real time RT-PCR 反應條件...23 4-1 LightCycler...25 5、核苷酸序列分析...28 參、實驗結果...30 1、Primer 和Probe 的設計...30 2、分型結果...
1. In this study, a rapid and specific TaqMan-based, one-step real-time quantitative reverse transcriptase PCR (qRT-PCR) is developed for the detection of peste des petits ruminants virus (PPRV). 建立了检测小反刍兽疫病毒的快速、特异的基于Taqman的一步法实时定量RT-PCR方法。
ABI公司7000型荧光定量PCR仪中文操作手册 VC 外源DNA残留量测定(荧光定量PCR法)标准操作规程SOP(实用应用文) ABI Stepone plus荧光定量PCR仪操作规程 "从RNA提取到荧光定量PCR"解决方案--qPCR系统 (精品课件)荧光定量PCR(Real Time PCR)实验原理,实验设计和结果分析介绍 AB7500荧光定量PCR仪使用维护校准标准操作规程完...
A novel real-time RT-PCR for influenza A(H1N1)v virus was set up ad hoc and validated followin... Marcus Panning,M Eickmann,O Landt,... - 《Eurosurveillance》 被引量: 312发表: 2009年 Development of a real-time RT-PCR for the detection of Swine-lineage Influenza A (H1N1) virus ...
本制品首先利用反转录酶 PrimeScript RTase 将 RNA 反转录成 cDNA ,再采用 TaqManTM探针法以cDNA为模 板利用TaKaRa Ex Taq HS在 同一反应管内连续进行 Real Time PCR扩增反应。 RT-PCR 反应采用了如图 1 显示 的 One Step RT-PCR 方法。反 转录反应以 RNA 为模板,以 Specific Primer(Reverse)为 反转录引物...
Protocol: SuperScript IV UniPrime One-Step RT-PCR System Brochure: Discover Superior RT-PCR results more easily than ever Application note: One-step RT-PCR protocol for amplicon-based viral genome sequencing Featured videos Learn how the SuperScript IV UniPrime One-Step RT-PCR System m...
StepOne型荧光定量PCR仪 操作规程: 1.StepOne上机可由两种方式来进行:由StepOne Software Quick Start进行上机或由StepOne机器面板直接触控上机。 2.由StepOne Software Quick Start进行上机: 开启StepOne Software v2.0进入主画面后点选Quick Start窗口进行快速上机设定。进入Experiment Properties后输入:Experiment ...
使用ABI 7900HT/ 7300 Real-Time PCR System和StepOne PlusTM 时使用50 × ROX Reference Dye 1 ;ABI 7500, 7500 Fast Real-Time PCR System,Stratagene Mx3000P使用50 × ROX Reference Dye 2 ;Roche, Bio-Rad的Real Time PCR仪时不必使用ROX。 储存条件 ® -20℃保存。2 × One Step SYBR Green ...