ABI荧光PCR仪Step one 英文名称: 总访问: 4301 国产/进口: 进口 半年访问: 295 产地/品牌: 美国/Thermo Fisher 产品类别: 定量PCR 型号: Step one 最后更新: 2024-12-17 货号: 4376357 参考报价: 160000 立即询价 电话咨询 [发表评论] [本类其他产品] [本类其他供应商] [收藏] 分享:微信新浪微博销售...
仪器有个自带的默认程序，是个两步法的程序，如果你用的也是ABI的SYBR green mix，可以直接用这个默认的程序，只需要修改一下60度的时间，一般这个仪器是在延伸那一步收集荧光，全板收集完差不多需要30秒，所以不论是两步法还是三步法，你的延伸那一步时间都不要少于30秒，其他的条件看你自己设计的引...
A one-step real time RT-PCR method for microRNA detection is described. The detection process includes reverse transcription and real time PCR. The whole process is performed by a one-step operation on a real-time PCR system. The method is low cost, rapid, sensitive and specific. The ...
ABI Step One Plus PCR仪全貌如图1,需搭配Step One Software软件使用(工程师会预设软件在电脑上)。 图1 ABI Step One Plus仪器 图2 仪器开机页面 二、使用方法 注意: 本操作指南仅针对SYRB染料法的操作,且本仪器必须使用含ROX的qPCR试液,推荐使用含High Rox的染料。 机器反应舱标配pcr管为100μl,通常配制试剂...
We developed a one-step Taqman-based real-time quantitative RT-PCR (RT-qPCR) assay targeting the conserved region of the 879 bp long full-length WYMV coat protein gene. The accuracy of normalized data was analyzed along with appropriate internal control genes: β-actin and 18s rRNA which ...
In this study, we developed a TaqMan probe-based, one-step real-time reverse transcriptase-polymerase chain reaction (RT-PCR) system for detection and serotyping DENV. Our linear dynamic range (101 to 107 copies/reaction) showed the R2 values of DENV-1, 2, 3 and 4 as 0.998, 0.998, ...
In this study, a rapid and specific TaqMan-based, one-step real-time quantitative reverse transcriptase PCR (qRT-PCR) is developed for the detection of peste des petits ruminants virus (PPRV). Primers and probe were designed based on the nucleocapsid protein gene sequence. The specificity of ...
本制品首先利用反转录酶 PrimeScript RTase 将 RNA 反转录成 cDNA ,再采用 TaqManTM探针法以cDNA为模 板利用TaKaRa Ex Taq HS在 同一反应管内连续进行 Real Time PCR扩增反应。 RT-PCR 反应采用了如图 1 显示 的 One Step RT-PCR 方法。反 转录反应以 RNA 为模板,以 Specific Primer(Reverse)为 反转录引物...
StepOne型荧光定量PCR仪 操作规程: 1.StepOne上机可由两种方式来进行:由StepOne Software Quick Start进行上机或由StepOne机器面板直接触控上机。 2.由StepOne Software Quick Start进行上机: 开启StepOne Software v2.0进入主画面后点选Quick Start窗口进行快速上机设定。进入Experiment Properties后输入:Experiment ...
Combination of highly processive enzymes in the SuperScript IV UniPrime One-Step RT-PCR System allows for the amplification of a broad range of target lengths (Figure 5). Full length cDNA is synthesized in as few as 10 min, and the PCR step requires an extension time of only 30...