一、仪器及软件介绍 ABI Step One Plus PCR仪全貌如图1,需搭配Step One Software软件使用(工程师会预设软件在电脑上)。 图1 ABI Step One Plus仪器 图2 仪器开机页面 二、使用方法 注意: 本操作指南仅针对SYRB染料法的操作,且本仪器必须使用含ROX的qPCR试液,推荐使用含High Rox的染料。 机器反应舱标配pcr管为...
Hieff Clone® Plus Multi One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品,该试剂盒可以将PCR产物定向克隆至任何载体的任何位点,可高效克隆50 bp-10 kb片段。将载体线性化,并在插入片段正、反向PCR引物5’端引入15-25 bp的线性化载体末端同源序列,使得
Hieff Clone® Plus One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品,该试剂盒可以将PCR产物定向克隆至任何载体的任何位点,可高效克隆50 bp-10 kb片段。将载体线性化,并在插入片段正、反向PCR引物5’端引入15-25 bp的线性化载体末端同源序列,使得插入片
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