Meanwhile, the medium was collected and used for evaluation of secretion of SDF-1α and TGF-β cytokines with enzyme-linked immunosorbent assay (ELISA) kits (mlbio, China) following the manufacturer’s guidance. Functional assessment of stem cells Mitochondrial activity of BMSCs BMSCs were ...
Primers and probes for osteogenic and adipogenic genes were obtained from TaqMan Gene Expression Assay Scientific Reports | 5:18279 | DOI: 10.1038/srep18279 2 www.nature.com/scientificreports/ kits (Applied Biosystems). Transcript levels were normalized to the housekeeping gene glyceraldehyde 3-...
as microglia-like cells and used for functional or biochemical assays. Cells were collected every 2 to 3 days based on the confluency and plated on PLL coated plates in near homeostatic medium with freshly added growth factors, for functional assays. The composition of near homeostatic medium is ...
c-Kit+ cells were isolated using mouse CD117 microbeads together with OctoMACS Separator and Starting kits (Miltenyi Biotec) following the manufacturer’s instructions. Induced deletion of Nat10 in vitro and in vivo For in vitro deletion of Nat10 in BM cells from Nat10fl/flCreERT2 mice, 1...
Inhibitor of DNA binding (ID) proteins are transcriptional regulators that control the timing of cell fate determination and differentiation in stem and progenitor cells. The ability of ID proteins to function as central 'hubs' for the coordination of mu
Narva E, Autio R, Rahkonen N, Kong L, Harrison N, Kitsberg D, Borghese L, Itskovitz-Eldor J, Rasool O, Dvorak P: High-resolution DNA analysis of human embryonic stem cell lines reveals culture-induced copy number changes and loss of heterozygosity. Nature Biotechnology. 2010, 28: 371-37...
After 12 h of cell culture, the upper chamber was separately incubated with PBS, GelMA, ABs, and AB@GMSs for 3 days and treated with live-dead cell staining kits for 30 min on days 1 and 3. The cells were observed by a fluorescence microscope. The live-dead staining results showed ...
a, A 3.2-mm keratome was used to make a limbus tunnel incision at the 11–12 o’clock position into the anterior chamber.b, The capsular opening was created by a capsulorhexis needle.c, A 1–2 mm diameter anterior capsulotomy was performed using the anterior continuous curvilinear capsu...
LA Shin: So if you [?] the current is going this way, so if you look at the left loop, it’s like this right? But then what if it like goes like this? So what’s going to happen? [arm movements] [laughs] It’s also hard for me. ...
In this study, we found that ectopic induction of the AP2/ERF transcription factor STEMIN1 in leaf cells of the moss Physcomitrella patens decreases a repressive chromatin mark, histone H3 lysine 27 trimethylation (H3K27me3), on its direct target genes before cell division, resulting in the ...