Stem cell culture is a crucial cell culture technique that involves diverse disciplines such as modern biotechnology. It is mainly used in the area of human health and diagnosis. The current advancement in stem cell culture technique leads to recognition of different types of stem cells such as ...
Cell Culture TechniquesCell DifferentiationSpermatogenesisStem CellsMaleMale germline stem cells--spermatogonial stem cells (SSCs)--self-renew and produce large numbers of differentiating germ cells that become spermatozoa throughout postnatal life and transmit genetic information to the next generation. SSCs...
Conventional two-dimensional (2D) cell culture techniques may undergo modifications in the future, as life scientists have widely acknowledged the ability of three-dimensional (3D) in vitro culture systems to accurately simulate in vivo biology. In recent years, researchers have discovered that microgra...
Administration of a single type of cell population such as stem cells or chondrocytes does not guarantee a full recovery of cartilage defects. Therefore, current tissue engineering approaches using co-culture techniques have been developed to mimic complex and dynamic cellular interactions in native ...
In the last 2 decades, cell culture techniques for both mammalian embryonic stem cells and adult stem cells have developed and improved, and are now widely available. These stem cells are either pluri- or multi-potent, which makes them favorable for use in vitro developmental toxicity assays. ...
Administration of a single type of cell population such as stem cells or chondrocytes does not guarantee a full recovery of cartilage defects. Therefore, current tissue engineering approaches using co-culture techniques have been developed to mimic complex and dynamic cellular interactions in native ...
‘Humanized’ stem cell culture techniques: the animal serum controversy. Stem Cells Int. 2011;2011:504723. Article PubMed Central PubMed Google Scholar Choi J, Chung JH, Kwon GY, Kim KW, Kim S, Chang H. Effectiveness of autologous serum as an alternative to fetal bovine serum in ...
[123,126,127,130]. All of these approaches use standard cell culture techniques and basic laboratory equipment making non-genetic-transdifferentiation of monocytes an accessible and inexpensive (Table1) alternative to obtain neuronal-like cells directly from patients. The disadvantages of generating ...
Cell culture is one of the most common and most complex techniques used in life sciences, andculture medium is a key factor for maintaining the health and proliferation of mesenchymal stem cells (MSCs).When completely broken down, all MSCs culture media essentially contain the same basic component...
While culture medium and systems have been described that permit the culture and proliferation of human embryonic stem cells in feeder free and animal product free conditions, these conditions will not readily support cloning of an embryonic stem cell culture meaning, at least here, the initiation ...