A process for preparing a conditioned cell culture medium is provided. The process comprises a) culturing eukaryotic cells in a growth medium having a composition effective to support cell growth; b) separating the cultured cells from the growth medium; and c) maintaining the cultured cells in a...
An Introduction to Stem Cell Maintenance Feeder-Dependent Culture of Human Embryonic Stem Cells (hESCs) Feeder-Dependent Culture of Human Induced Pluripotent Stem Cells (hiPSCs) Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium Culturing ...
Mesenchymal stem cell conditioned medium 专利名称：Mesenchymal stem cell conditioned medium 发明人：Sai Kiang Lim,Elias Lye 申请号：US12377398 申请日：20070815 公开号：US09029146B2 公开日：20150512 专利内容由知识产权出版社提供 摘要：We disclose a method of preparing a conditioned cell culture medium, ...
We evaluated the therapeutic potential of mesenchymal stem cell-conditioned medium (CM-MSC) as an alternative to cell therapy in an antigen-induced model of arthritis (AIA). Disease severity and cartilage loss were evaluated by histopathological analysis of arthritic knee joints and immunostaining of ...
“stem cell” and “conditioned medium” or “secretome” and “therapy.” Data concerning treated conditions/diseases, type of cell that was cultured, medium and supplements to culture the cells, culture condition, CM processing, growth factors and other secretions that were analyzed, method of ...
In recent years, stem cell therapy has become a very promising and advanced scientific research topic. The development of treatment methods has evoked great expectations. This paper is a review focused on the discovery of different stem cells and the pot
Human mesenchymal stem cell-conditioned medium improves cardiac function following myocardial infarction. Stem Cell Res 2011; 6: 206–214. PubMed Google Scholar Van Overstraeten-Schlögel N, Beguin Y, Gothot A . Role of stromal-derived factor-1 in the hematopoietic-supporting activity of human...
Also, the exosomes from conditioned medium of urine-derived stem cells (USCs-Exo) might have the potential to prevent kidney injury from diabetes by inhibiting podocyte apoptosis and promoting vascular regeneration and cell survival [194]. Since these cells could be conveniently obtained through non-...
cell infiltration was found in the peri-wound area (data not shown). In light of the beneficial effects of the conditioned medium in vitro, we modified the delivery method according to previously published protocols [12]. In Sun et al.’s study, MSC-conditioned medium-hydrogel was pipetted ...
Objectives Wound healing is a complex process with a sequence of restoring and inhibition events such as cell proliferation, differentiation, migration as well as adhesion. Mesenchymal stem cells (MSC) derived conditioned medium (CM) has potent therapeut