Since you’ve replaced so much, the only likely possibilities left is that there is a clog in the high pressure line, there is air leaking into the low pressure line or the replacement pump you installed is damaged or not correct for your vehicle. Try checking the high pressure line for ...
but if I hold the outer portion at the rear the only movement I get is from the clips moving in the frame. Im trying to avoid having to disasemble the wheel twice but cant decide if the angle sensor on the back of the replacement is any good. There is a line that has been drawn...
the column clicks very badly. If I go over a bump or railroad track it clicks/rattles very badly. We had the CV Joints replaced on both sides last year, So I know this can’t be the problem. Can you give me any idea what the problem can be?
Pooled fractions were diluted 1:1 with 20 mM b-mercaptoethanol and loaded onto a 5 ml HiTrap Q FF column to remove nucleic acid contamination, with a 20 CV elution gradient from 0 to 1.0 M NaCl in 20 mM Tris pH 8.0, 1 mM EDTA, 0.02% NaN3, 20 mM b-mercaptoethanol. The flow-...
(250 mM imidazole pH 7.2, 0.5 M NaCl, 0.02% NaN3, 5 mM β-mercaptoethanol). Pooled fractions were diluted 1:5 with water and then loaded onto a HiPrep Heparin FF 16/10 column. The column was washed with 5 CV buffer HEP-A1 (25 mM Tris pH 7.5, 1 mM CaCl2, 0.02% ...