(New England Biolabs Inc., Ipswich, Mass.) can extend primers on immobilized DNA (FIG. 9B). One can perform polymerase extension to, for example, about 400 bases. The extension fragments can then be collected, a library constructed, and the library and barcode sequenced using standard ...
and the pNEB193 vector (New England BioLabs, Inc.; Ipswich, MA). pCB1 encodes a cyclin B 1-specific small hairpin RNA (shRNA) and a Zoanthus sp. green fluorescent protein (ZsGreen). Constitutive shRNA expression is driven by the RNA Pol III-dependent human U6 promoter (PU6), while ...