The reaction mixture is then left for 48 hours (woth stirring) at 25° C. After this time the mixture is poured into an ice-cold 20% hydrochloric acid solution and stirred for 30 mins. The product is extracted into ether and the combined extract is washed with water and dried (Na2SO4...
In some embodiments, the feruloyl-CoA:monolignol transferase nucleic acid encodes a feruloyl-CoA:monolignol transferase polypeptide with a SEQ ID NO:2 sequence. In other embodiments, the nucleic acids can, for example, encode a feruloyl-CoA:monolignol transferase that can catalyze the synthesis of ...