The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot. The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent). ...
System TypeWestern Blotting, SDS-PAGE Unit SizeEach 內容物與存放 Contents: 250 μL of HiMark™ Pre-Stained HMW Protein StandardStorage Buffer: Tris-HCl, Formamide, SDS, Phenol RedStorage:Store at -20°C Introducing iBlot 3 Western Blot Transfer System Featuring higher throughput and built-in...
The following protocol uses a tank blotting system and a horseradish peroxidase-conjugated secondary antibody of an enhanced chemoluminescence (ECL) detection system (GE Healthcare) Fig. 3. Setup of gel/membrane blotting sandwich for western blot for revealing antigen-antibody complexes: transfer of ...
P7712, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
et al. A double-blind clinical trial to compare the efficacy and safety of a multiple amino acid-based ORS with the standard WHO-ORS in the management of non-cholera acute watery diarrhea in infants and young children: “VS002A” trial protocol. Trials 23, 706 (2022). https://doi.org...
Western analysis Total protein was extracted from described ovarian cell cultures expressing Caspase 8 or scrambled shRNA untreated, treated with chemotherapy, and/or birinapant using RIPA buffer (sc-24948) according to the manufacturer’s protocol (Santa Cruz Biotechnology, Santa Cruz, CA) and concen...
a Western blot analysis and quantification of full-length 6TM TRPM8 amount in RWPE-1 and LNCaPFGC cell lines expressing endogenous (WT), overexpressed (M8) or knocked-out (CAS) levels of the protein. b Immunofluorescence analysis and quantification of PM-associated full-length 6TM TRPM8 in ...
Western blot revealed adequate presence of the known prostate specific markers, PSA and PAP, demonstrating that prostate specific proteins are sufficiently present in a standard, non-post-DRE urine specimen for biomarker analysis. Preliminary Western Blot analysis demonstrated elevated expression of a 23...
System TypeWestern Blotting, SDS-PAGE Unit SizeEach 內容物與存放 500 μL (50 applications of 10 μL each) provided in a plastic vial. Loading Buffer consists of Tris-HCl, Formamide, SDS, and Phenol Red. Store at 4°C. Introducing iBlot 3 Western Blot Transfer System ...
Our total detection range using this protocol spans over 2 orders of magnitude, but the quantifiable range is narrower. Quantitative western blot: detailed method description. ||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn...