the best standard is one that will produce a normal (i.e., average) color response curve with the selected protein assay method and is readily available to any researcher. BSA is such a protein, and the Pierce Albumin Standards are the most convenient source of ready-to-use BSA standard....
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Ten kinds of protein standards were analyzed using PROTEIN KW-803 (a column for aqueous SEC (GFC)). Sample : 1. Thyroglobulin (MW: 669,000) 2. Aldolase (MW: 158,000) 3. BSA (MW: 67,000) 4. Ovalbumin (MW: 43,000) 5. Peroxidase (MW: 40,200) 6. Adenylate kinase (MW: 32,...
Should a standard curve for a Lowry protein assay using a BSA standard look identical to a standard curve based on lysozyme? Explain why or why not. How does macroamylase affect the amylase/creatinine clearance ratio? Explain how this serological test is used: Enzyme-linked immunosorbent...
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curve overlays the signal response curve of the spiked biological test sample when evaluating the total analyte concentration. High fold-dilutions of the test sample may nullify matrix interference if the immunoassay affords sufficient sensitivity. However, many proteins of clinical interest (e.g. ...
DNA concentration was measured via Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and DNA amount was calculated based on the concentration and volume of each sample. Absolute vector copies were measured in each sample using a standard curve and qPCR primer probe set designed to recognize the ...
Methods A multicenter randomized (1:1) controlled trial was performed to assess superiority of optimized dosing versus BSA-based dosing in patients who were eligible for pemetrexed-based chemotherapy. The individual exposure to pemetrexed in terms of area under the concentration–time curve (AUC) was...
Per experiment and condition, impedance of 5–7 electrodes was analysed. Full details are provided in the Supplementary Methods. Statistical analysis All statistical analyses were performed using Graphpad Prism 5.02. IC50 values were determined by nonlinear sigmoidal curve fitting with normalised response ...
Prepare a convenient standard curve using a solution 1 mg/ml phenylalanine residues. of bovine serum albumin (BSA fraction V) in either isotonic 1. Prepare the protein reagent (Bradford reagent) by dissolving 100 mg saline or, in the case of any possibly interfering substance of Coomassie ...