In lab we will use Mycobacterium smegmatis (M. smeg), which is a non-pathogenic bacterium in this genus. Mycobacteria have a cell wall that is different from both Gram-negative and Gram-positive bacteria. Mycobacteria can stain either Gram-positive or Gram-negative depending on the age of ...
Gram Negative Lab Report From one of my modified streak plates I was able to successfully isolate my Gram positive bacteria. By my 6th plate, there was a distinction between my Gram positive and Gram negative in morphology. To make sure it was absolutely pure I continued to streak it two ...
In contrast to direct stains that bind to bacteria directly, a negative stain colors the background of a smear rather than the bacteria. These stains have negatively charged functional groups so they will not bind directly to negatively charged bacteria. The advantages of negative staining are: 1...
Fig. 2. Stained images from a lab-scale AS system using bright-field microscopy with 1000× total magnification. (a) Gram staining. Arrow shows a typical Gram negative filamentous bacteria (in red) (b) Neisser staining. Arrows show a Neisser positive filamentous bacteria (in purple) and possi...
An endospore is anon-vegetative structureproduced by a group of bacteria belonging to the Firmicute family. They have special characteristics that stabilize them to survive in adverse conditions for long periods of time. These include; a phase of dormancy, a tough outer covering and they are non...
Gram positive bacteria produces a blue/purple color with this staining procedure while gram negative ones are red in color. REAGENTS/STORAGE: Reagent kits manufactured by BBL and Difco can be purchased from a local scientific supply house and contains four reagent bottles: Crystal violet -- the...
Staining Solutions for Simple Stains of Bacteria - Staining Solutions Staining Solutions for Bacteria and Bovine Cells in Milk - Staining Solutions Staining Solutions for Blood Films for Spirochetes, Protozoa and other purposes - Staining Solutions ...
in sections of formalin-fixed, paraffinembedded (FFPE) tissue stained on the BenchMark Special Stains instrument.This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.This product is intended for in ...
A simple apparatus and method is disclosed for making individual single Gram stains on bacteria inoculated slides to assist in classifying bacteria in the laboratory as Gram-positive or Gram-negative. The apparatus involves positioning a single inoculated slide in a stationary position and thereafter ...
Bacteria and fungus can grow in stainers and reagent vessels if these areas are not regularly cleaned. Follow the manufacturer instructions for instrument maintenance. Remember that while bleach is appropriate for most things, it may cause issues with your instrument. ...