Therefore, we used the C-terminal biotin-tag on ConC gp140 trimers to capture Env on Streptavidin-coated plates and hence maintained a more native-like trimer conformation in a capture coat ELISA. The antibody response to conformational epitopes was similar for all three groups, with titers of...
Total conjugated trimer was quantified by ELISA in the presence of 1% triton-X and 100 mM imidazole to fully disrupt liposomes and Ni-6xHis interactions, respectively. Trimer was captured on Nunc MaxiSorp plates coated with 2 µg/ml (mouse Fc) VRC01 and detected using 0.2 µg/m...