Next, the membranes were washed 3 times with TBS-T followed by incubating with HRP-conjugated goat anti-rabbit IgG or HRP-conjugated goat anti-mouse IgG for 1 h. After washing 3 times with TBS-T, an enhanced chemiluminescence (ECL) detection system (Bio-Rad, Hercules, CA, USA) was used...
Cells were plated 24 h before treatment. Recombinant rat secretory ST6GAL1 enzyme (rST6G) [24] was produced by Dr. Kelley W. Moremen, University of Georgia, Georgia, USA. CMP-sialic acid (CMP-Sia)(MilliporeSigma, Massachusetts, USA), DAPI (BioLegend, CA, USA; 0.5 µg/ml), ...
(W = side to side) (D = front to back) (H = top to bottom) Note:Units 84" and Up Work Tables are Furnished with (6) Legs Finished Size of Undershelf:Shelf Width = Width Minus 4-7/8" and Shelf Depth = Depth Minus 4-1/4" ...
Expression of the H type 1 blood group antigen during enterocytic differentiation of Caco-2 cells. J Biol Chem 1999;274:21209–21216. 22. Brockhausen I, Romero PA, Herscovics A. Glycosyltransferase changes upon differentiation of CaCo-2 human colonic adenocarcinoma cells. Cancer Res 1991;51:...
Cells were then plated into 96 well cell culture plates (5 × 103 cells/well for cell proliferation assay and SNA staining; 5 × 104 cells/well for scratch wound healing assay and 1 × 104 cells/well for apoptosis assays; 8 wells/each assay). 8 h later, neuraminic...
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On the next day, the membranes were washed with TBST 3 times before incubation with specific secondary antibodies for 1 h at room temperature. Signals were detected by using a standard ECL kit. The primary antibodies used in the study were as follows: anti-SP1 (1:2000; Cell Signalling, ...
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(BHA, Scharlab) dishes using a bacterial suspension adjusted to 1 × 106CFU/mL to form a bacterial lawn. Once the plate was dried, drops of 10 µL of different isolated strains. After incubation at 30 °C for 48 h, the plates were examined to determine the absence/presence of ...
Cells were then washed with PBS three times and incubated with Alexa Fluor 633 anti-mouse antibody (Invitrogen, Carlsbad, CA, USA) at a 1:200 dilution for 1 h. After three PBS washes, cells were counterstained with 4',6'-diamidino-2-phenylindole for 5 min and analyzed using an Olympus...