Texas Wheat, from St. Arnold
Mytomycin C (Sigma-Aldrich, St. Louis, MO) was added to a final concentration of 0.1 μg/ml and the culture was incubated until lysis occurred, as shown by a decrease in turbidity. Cellular debris was pelleted at 16000 g for 15 min. The induced supernatant was filtered through a 0.44...