ssDNA reporter (FAM-TTTTT-BHQ1) 仅供科研使用,不可以应用于人及动物的治-疗或诊断。 公司简介 武汉科斯坦生物科技有限公司是一家致力于前沿生物技术研发与成果转化的创新型高科技企业。 公司成立以来,一直专注于生命科学研发与技术服务领域,与 高校建立合作关系,逐步建立并完善了“分离纯化平台”、“分析检测平台”...
Schematic representation of ssDNA mutagenesis reporter system.Kin, ChanJoan, F. SterlingSteven, A. RobertsAshok, S. BhagwatMichael, A. ResnickDmitry, A. Gordenin
近些年,crispr (clustered regularlyinterspaced short palindromic repeats)系统反式切割性质的发现促使基于crispr的分子诊断方法迅速发展,如张锋团队基于cas13a开发出的sherlock (specific highsensitivity enzymatic reporter unlocking),jennifer doudna团队基于cas12a、cas14开发的detectr (dna endonuclease-targeted crispr trans...
(HyClone) and 1% penicillin–streptomycin stock (penicillin 50 U/ml and streptomycin 50 μg/ml, final concentration; Gibco). TZM-bl cells contain a HIV-1 tat-inducible luciferase reporter gene that correlates with HIV-1 infectivity. All cells were maintained in humidified 37 °C ...
Link net Reporter Li Zong Ze reported that, according to US Los Angeles Times on May 27 the news, middle Syria the Holmes province shook off the townspeople area to suff[translate] a在代表着美丽纯净的无花果树下开始他们甜蜜的初恋 In represents under the beautiful pure blossomless fruit tree to...
We constructed a single-copy, genomically integrated blue fluorescent protein (BFP) reporter cell model to assess the potency of ssDNA donor for mediating HDR, which can implement BFP to green fluorescence protein (GFP) conversion when the 66th amino acid “His” in BFP is replaced by amino ...
632666Guide-it™ Long ssDNA Production System v250 Rxns* The Guide-it Long ssDNA Production System v2 includes all the reagents and materials necessary for on-demand preparation of single-stranded DNA (ssDNA) repair templates of any sequence up to 5,000 nt in length for engineering gene knock...
The BBC assay reporter ssDNA can be modified for rapid readout. Two ssDNA molecules are designed to co-polymerize and then continually hybridize into double stranded DNA. The double stranded DNA can be optically and/or electrically detected. Kits related to the two ssDNA molecules and the BBC ...
DNA-protein interactions are commonly studied by using nitrocellulose filter-binding assays,DNA foot-printing,gel-shift assays,ELISA,or yeast reporter systems.Jin Kiat NgParayil Kumaran AjikumarGregory StephanopoulosChembiochem: A European journal of chemical biology...
Mutations were scored at many sites in the lacZ伪 mutation reporter sequence. Unexpectedly, the accessory proteins sometimes decreased and sometimes increased fidelity at a handful of specific sites. Here, we enlarge our analysis with one particular mutator polymerase compromised in both insertion ...